The study by Galanos, Freudenberg, and Reutter investigates the effect of D-galactosamine on the sensitivity of rabbits, rats, and mice to the lethal effects of lipopolysaccharide (LPS). Treatment with galactosamine significantly increased the animals' susceptibility to LPS, with the highest sensitivity observed when LPS was injected within 1-3 hours after galactosamine. The onset of lethality was faster in animals treated with both substances, typically occurring 5-9 hours later. The sensitization could be reversed by injecting galactosamine within 1 hour after LPS injection. Biochemical alterations in hepatocytes induced by galactosamine were found to be responsible for the increased sensitivity to LPS. The study suggests that the early metabolic effects of galactosamine, rather than the later cellular damage, are crucial in this process. This model provides a useful tool for studying the mechanisms of endotoxin toxicity.The study by Galanos, Freudenberg, and Reutter investigates the effect of D-galactosamine on the sensitivity of rabbits, rats, and mice to the lethal effects of lipopolysaccharide (LPS). Treatment with galactosamine significantly increased the animals' susceptibility to LPS, with the highest sensitivity observed when LPS was injected within 1-3 hours after galactosamine. The onset of lethality was faster in animals treated with both substances, typically occurring 5-9 hours later. The sensitization could be reversed by injecting galactosamine within 1 hour after LPS injection. Biochemical alterations in hepatocytes induced by galactosamine were found to be responsible for the increased sensitivity to LPS. The study suggests that the early metabolic effects of galactosamine, rather than the later cellular damage, are crucial in this process. This model provides a useful tool for studying the mechanisms of endotoxin toxicity.