Galaxy is a web-based genome analysis tool designed to support high-throughput data analysis, particularly for experimentalists without informatics or programming expertise. It provides a user-friendly interface for performing complex, large-scale analyses, while automatically managing computational details. Galaxy is available as a public web service and as a downloadable package for individual labs. The tool supports various genomic data types, including interval data, and offers features such as joining, grouping, sorting, and filtering. This article introduces Galaxy through a series of protocols, demonstrating how to use it for tasks such as finding promoters containing TAF1 binding sites from CHiP-seq data, identifying exons with the highest number of SNPs, saving and sharing analyses, generating workflows, and extracting genomic sequences and alignments. Galaxy facilitates reproducible and collaborative research by preserving all analysis details in a "history" that can be shared or published.Galaxy is a web-based genome analysis tool designed to support high-throughput data analysis, particularly for experimentalists without informatics or programming expertise. It provides a user-friendly interface for performing complex, large-scale analyses, while automatically managing computational details. Galaxy is available as a public web service and as a downloadable package for individual labs. The tool supports various genomic data types, including interval data, and offers features such as joining, grouping, sorting, and filtering. This article introduces Galaxy through a series of protocols, demonstrating how to use it for tasks such as finding promoters containing TAF1 binding sites from CHiP-seq data, identifying exons with the highest number of SNPs, saving and sharing analyses, generating workflows, and extracting genomic sequences and alignments. Galaxy facilitates reproducible and collaborative research by preserving all analysis details in a "history" that can be shared or published.