GeneCards, a comprehensive and authoritative resource for human gene information, has introduced its third version (V3). This update features a sophisticated search engine and an enhanced infrastructure to meet the growing needs of biomedical researchers. Key improvements include gene-set analyses, such as GeneAlaCart for batch query facilities and GeneDecks for identifying similar genes with shared annotations. The new version also includes expanded visualization of gene expression patterns, integrated alternative splicing patterns, and augmented multi-source SNP and pathway sections. GeneCards now provides direct links to research reagents and gene-related drugs, and introduces the GeneCards Inferred Functionality Score (GIFTS) annotation landscape tool. The article highlights the new database architecture, search engine capabilities, and semi-automated quality assurance system. Examples of applications and collaborations that have benefited from GeneCards are provided, emphasizing its role in facilitating systems biology efforts and drug targeting.GeneCards, a comprehensive and authoritative resource for human gene information, has introduced its third version (V3). This update features a sophisticated search engine and an enhanced infrastructure to meet the growing needs of biomedical researchers. Key improvements include gene-set analyses, such as GeneAlaCart for batch query facilities and GeneDecks for identifying similar genes with shared annotations. The new version also includes expanded visualization of gene expression patterns, integrated alternative splicing patterns, and augmented multi-source SNP and pathway sections. GeneCards now provides direct links to research reagents and gene-related drugs, and introduces the GeneCards Inferred Functionality Score (GIFTS) annotation landscape tool. The article highlights the new database architecture, search engine capabilities, and semi-automated quality assurance system. Examples of applications and collaborations that have benefited from GeneCards are provided, emphasizing its role in facilitating systems biology efforts and drug targeting.