GeneMANIA is a web-based tool for generating hypotheses about gene function, analyzing gene lists, and prioritizing genes for functional assays. It uses a wealth of genomics and proteomics data to find functionally similar genes and predict gene function based on interactions. It supports nine organisms and has access to hundreds of data sets and millions of interactions. The user interface has been redesigned to be more intuitive and efficient, with features such as a new layout, improved gestures, and a query history. The Cytoscape app has been updated to integrate with GeneMANIA, allowing for live queries and analysis. New organisms, such as zebrafish and E. coli, have been added, along with new data sources. The tool now supports network search, allowing users to select specific networks and view connections among query genes. The report generation functionality has been improved, with larger figures and direct PDF output. Future updates include a network search feature and integration with Enrichment Map pathway analysis. Funding comes from various sources, including the Ontario Global Leadership Round in Genomics and Life Sciences and US NIH grants. The authors declare no conflict of interest.GeneMANIA is a web-based tool for generating hypotheses about gene function, analyzing gene lists, and prioritizing genes for functional assays. It uses a wealth of genomics and proteomics data to find functionally similar genes and predict gene function based on interactions. It supports nine organisms and has access to hundreds of data sets and millions of interactions. The user interface has been redesigned to be more intuitive and efficient, with features such as a new layout, improved gestures, and a query history. The Cytoscape app has been updated to integrate with GeneMANIA, allowing for live queries and analysis. New organisms, such as zebrafish and E. coli, have been added, along with new data sources. The tool now supports network search, allowing users to select specific networks and view connections among query genes. The report generation functionality has been improved, with larger figures and direct PDF output. Future updates include a network search feature and integration with Enrichment Map pathway analysis. Funding comes from various sources, including the Ontario Global Leadership Round in Genomics and Life Sciences and US NIH grants. The authors declare no conflict of interest.