The article describes a protocol for generating 3D brain tissue, known as cerebral organoids, from human pluripotent stem cells (hPSCs). This method mimics the endogenous developmental program and can be easily implemented in a standard tissue culture room. The protocol involves several key steps: forming embryoid bodies (EBs) from hPSCs, inducing neural differentiation, and promoting 3D spatial organization. The resulting organoids can develop into various brain structures, including the cerebral cortex, ventral telencephalon, choroid plexus, and retina, within 1–2 months. The organoids can be maintained for over 1 year, allowing for the study of neuronal maturation and survival. The method is particularly useful for studying human brain development and diseases, such as neurodevelopmental disorders and neurological conditions. The article also compares this method with other approaches, highlighting its advantages in terms of complexity and organization.The article describes a protocol for generating 3D brain tissue, known as cerebral organoids, from human pluripotent stem cells (hPSCs). This method mimics the endogenous developmental program and can be easily implemented in a standard tissue culture room. The protocol involves several key steps: forming embryoid bodies (EBs) from hPSCs, inducing neural differentiation, and promoting 3D spatial organization. The resulting organoids can develop into various brain structures, including the cerebral cortex, ventral telencephalon, choroid plexus, and retina, within 1–2 months. The organoids can be maintained for over 1 year, allowing for the study of neuronal maturation and survival. The method is particularly useful for studying human brain development and diseases, such as neurodevelopmental disorders and neurological conditions. The article also compares this method with other approaches, highlighting its advantages in terms of complexity and organization.