The study describes a method to generate human induced pluripotent stem (iPS) cells from dermal fibroblasts by expressing defined transcription factors KLF4, OCT4, SOX2, and C-MYC. The resulting iPS cells are morphologically and functionally indistinguishable from human embryonic stem (HESC) cells, sharing a nearly identical gene-expression profile. DNA fingerprinting and karyotypic analyses confirm that the iPS cells are derived from the donor material and do not undergo chromosomal abnormalities. The iPS cells can differentiate into lineages representative of the three embryonic germ layers, demonstrating their pluripotency. This approach provides a promising method for generating patient-specific pluripotent stem cells for clinical applications.The study describes a method to generate human induced pluripotent stem (iPS) cells from dermal fibroblasts by expressing defined transcription factors KLF4, OCT4, SOX2, and C-MYC. The resulting iPS cells are morphologically and functionally indistinguishable from human embryonic stem (HESC) cells, sharing a nearly identical gene-expression profile. DNA fingerprinting and karyotypic analyses confirm that the iPS cells are derived from the donor material and do not undergo chromosomal abnormalities. The iPS cells can differentiate into lineages representative of the three embryonic germ layers, demonstrating their pluripotency. This approach provides a promising method for generating patient-specific pluripotent stem cells for clinical applications.