The DPPH method is a widely used technique for assessing antioxidant activity. It involves measuring the ability of antioxidants to scavenge the stable free radical DPPH (2,2-diphenyl-1-picrylhydrazyl). The method is simple, rapid, and cost-effective, making it suitable for various applications. The basic principle is that antioxidants reduce DPPH, causing a decrease in absorbance at 517 nm. The method has been modified over time to improve accuracy and adaptability to different sample types and conditions. The DPPH method has been used to evaluate antioxidant activity in various biological samples, including plant extracts, food products, and beverages. It has been adapted for use in different solvent systems and has been combined with other techniques such as HPLC, TLC, and electrochemical methods to enhance its capabilities. These modifications allow for more precise and efficient determination of antioxidant activity, including the use of automated systems and high-throughput screening methods. The method has also been used to quantify antioxidants in complex biological systems and to assess the antioxidant capacity of various compounds. However, it has limitations, such as sensitivity to certain solvents and pH values, and the potential for interference from other compounds. Despite these limitations, the DPPH method remains a valuable tool for antioxidant assessment due to its simplicity and reliability. It is important to consider the specific requirements of each application when using the DPPH method to ensure accurate and meaningful results.The DPPH method is a widely used technique for assessing antioxidant activity. It involves measuring the ability of antioxidants to scavenge the stable free radical DPPH (2,2-diphenyl-1-picrylhydrazyl). The method is simple, rapid, and cost-effective, making it suitable for various applications. The basic principle is that antioxidants reduce DPPH, causing a decrease in absorbance at 517 nm. The method has been modified over time to improve accuracy and adaptability to different sample types and conditions. The DPPH method has been used to evaluate antioxidant activity in various biological samples, including plant extracts, food products, and beverages. It has been adapted for use in different solvent systems and has been combined with other techniques such as HPLC, TLC, and electrochemical methods to enhance its capabilities. These modifications allow for more precise and efficient determination of antioxidant activity, including the use of automated systems and high-throughput screening methods. The method has also been used to quantify antioxidants in complex biological systems and to assess the antioxidant capacity of various compounds. However, it has limitations, such as sensitivity to certain solvents and pH values, and the potential for interference from other compounds. Despite these limitations, the DPPH method remains a valuable tool for antioxidant assessment due to its simplicity and reliability. It is important to consider the specific requirements of each application when using the DPPH method to ensure accurate and meaningful results.