The article provides a comprehensive review of the development and applications of the DPPH method for antioxidant assay. The DPPH method, introduced by Blois in 1958, uses the stable free radical α, α-diphenyl-β-picrylhydrazyl (DPPH) to evaluate the antioxidant activity of compounds, extracts, and biological sources. The method is simple, rapid, and inexpensive, making it widely used for assessing antioxidant capacity. The article discusses various modifications and advancements to the DPPH method, including the use of different solvents, automation techniques like flow injection analysis (FIA) and sequential injection analysis (SIA), and high-throughput methods. These modifications enhance the method's applicability to a wide range of samples and improve its efficiency. The article also highlights the limitations of the DPPH method, such as interference from light, oxygen, and pH, and suggests improvements to address these issues. Overall, the DPPH method remains a valuable tool for antioxidant research, but its application should be tailored to the specific requirements of the study.The article provides a comprehensive review of the development and applications of the DPPH method for antioxidant assay. The DPPH method, introduced by Blois in 1958, uses the stable free radical α, α-diphenyl-β-picrylhydrazyl (DPPH) to evaluate the antioxidant activity of compounds, extracts, and biological sources. The method is simple, rapid, and inexpensive, making it widely used for assessing antioxidant capacity. The article discusses various modifications and advancements to the DPPH method, including the use of different solvents, automation techniques like flow injection analysis (FIA) and sequential injection analysis (SIA), and high-throughput methods. These modifications enhance the method's applicability to a wide range of samples and improve its efficiency. The article also highlights the limitations of the DPPH method, such as interference from light, oxygen, and pH, and suggests improvements to address these issues. Overall, the DPPH method remains a valuable tool for antioxidant research, but its application should be tailored to the specific requirements of the study.