The complete genome sequence of the radiation-resistant bacterium *Deinococcus radiodurans* R1 has been determined, comprising two chromosomes (2,648,615 and 412,340 basepairs), a megaplasmid (177,466 basepairs), and a small plasmid (45,702 basepairs). The genome contains 3,193 open reading frames (ORFs), with an average size of 937 bp, representing 91% of the total genome. *D. radiodurans* is known for its extreme resistance to radiation, oxidative stress, and DNA damage, and the genome reveals multiple components that contribute to these survival mechanisms. Key features include a highly efficient DNA repair system, including nucleotide excision repair, base excision repair, mismatch repair, and recombinational repair. The genome also encodes a large number of DNA glycosylases, MutY-Nth proteins, and UvrAs, suggesting a high level of redundancy in DNA repair. Additionally, the presence of DNA repeat elements and the ability to export damaged nucleotides out of the cell may contribute to its unique resistance to DNA damage. The genome also contains genes involved in stress recovery, such as amino acid utilization, cell envelope formation, and transporters, which are likely essential for survival under extreme conditions. The distribution of regulatory genes on the smaller genetic elements suggests they may play a role in stress response regulation.The complete genome sequence of the radiation-resistant bacterium *Deinococcus radiodurans* R1 has been determined, comprising two chromosomes (2,648,615 and 412,340 basepairs), a megaplasmid (177,466 basepairs), and a small plasmid (45,702 basepairs). The genome contains 3,193 open reading frames (ORFs), with an average size of 937 bp, representing 91% of the total genome. *D. radiodurans* is known for its extreme resistance to radiation, oxidative stress, and DNA damage, and the genome reveals multiple components that contribute to these survival mechanisms. Key features include a highly efficient DNA repair system, including nucleotide excision repair, base excision repair, mismatch repair, and recombinational repair. The genome also encodes a large number of DNA glycosylases, MutY-Nth proteins, and UvrAs, suggesting a high level of redundancy in DNA repair. Additionally, the presence of DNA repeat elements and the ability to export damaged nucleotides out of the cell may contribute to its unique resistance to DNA damage. The genome also contains genes involved in stress recovery, such as amino acid utilization, cell envelope formation, and transporters, which are likely essential for survival under extreme conditions. The distribution of regulatory genes on the smaller genetic elements suggests they may play a role in stress response regulation.