The article presents the complete genomic sequence of *Borrelia burgdorferi*, the causative agent of Lyme disease. The genome consists of a linear chromosome of 910,725 base pairs and at least 17 linear and circular plasmids, totaling over 533,000 base pairs. The chromosome contains 853 genes encoding essential proteins for DNA replication, transcription, translation, solute transport, and energy metabolism, but lacks genes for cellular biosynthetic reactions, similar to *Mycoplasma genitalium*. The plasmids, which have a combined size of over 533,000 base pairs, contain mostly unknown genes, with 39% being paralogues forming 47 gene families. The biological significance of these genes is unclear but may be involved in antigenic variation or immune evasion. The genome analysis reveals a limited metabolic capacity, similar to *M. genitalium*, suggesting convergent evolution from more metabolically competent ancestors. The study also discusses the origin of replication, transcription, translation, DNA repair, and energy metabolism in *B. burgdorferi*. The complete genome sequence will aid in understanding the pathogenesis, prevention, and treatment of Lyme disease.The article presents the complete genomic sequence of *Borrelia burgdorferi*, the causative agent of Lyme disease. The genome consists of a linear chromosome of 910,725 base pairs and at least 17 linear and circular plasmids, totaling over 533,000 base pairs. The chromosome contains 853 genes encoding essential proteins for DNA replication, transcription, translation, solute transport, and energy metabolism, but lacks genes for cellular biosynthetic reactions, similar to *Mycoplasma genitalium*. The plasmids, which have a combined size of over 533,000 base pairs, contain mostly unknown genes, with 39% being paralogues forming 47 gene families. The biological significance of these genes is unclear but may be involved in antigenic variation or immune evasion. The genome analysis reveals a limited metabolic capacity, similar to *M. genitalium*, suggesting convergent evolution from more metabolically competent ancestors. The study also discusses the origin of replication, transcription, translation, DNA repair, and energy metabolism in *B. burgdorferi*. The complete genome sequence will aid in understanding the pathogenesis, prevention, and treatment of Lyme disease.