Since January 2020, Elsevier has created a free COVID-19 resource center with English and Mandarin information on the novel coronavirus. The center is hosted on Elsevier Connect, a public news and information website. Elsevier grants permission to make all its COVID-19-related research immediately available in PubMed Central and other public repositories for unrestricted use. This review discusses current advances in graphene bioapplications, focusing on biofunctionalization, biosensor development using graphene, and living cell studies. Graphene, a two-dimensional carbon material, has unique structural and electronic properties, making it a promising candidate for biotechnology. It can be used to create 0D fullerenes, 1D carbon nanotubes, and 3D graphite. Various methods have been developed to produce graphene, including micromechanical exfoliation, chemical vapor deposition, and chemical synthesis. Graphene and graphene oxide (GO) have been used in biological studies due to their unique properties, such as high surface area, mechanical strength, and thermal conductivity. They have been functionalized with DNA, proteins, and other biomolecules to create biosystems for various applications, including biosensors, drug delivery, and cell imaging. Graphene-based FRET biosensors have been developed for detecting DNA, ions, small molecules, and proteins. These sensors utilize the quenching properties of graphene and the recognition capabilities of biomolecules. Graphene-based nanomaterials have shown potential in biotechnology for applications such as drug delivery, cell imaging, and biosensing. However, challenges remain in controlling the size and number of graphene layers, ensuring biocompatibility, and understanding the cellular uptake and metabolic pathways of graphene and its derivatives. Future research should focus on improving the sensitivity and specificity of graphene-based biosensors and exploring new applications in biotechnology.Since January 2020, Elsevier has created a free COVID-19 resource center with English and Mandarin information on the novel coronavirus. The center is hosted on Elsevier Connect, a public news and information website. Elsevier grants permission to make all its COVID-19-related research immediately available in PubMed Central and other public repositories for unrestricted use. This review discusses current advances in graphene bioapplications, focusing on biofunctionalization, biosensor development using graphene, and living cell studies. Graphene, a two-dimensional carbon material, has unique structural and electronic properties, making it a promising candidate for biotechnology. It can be used to create 0D fullerenes, 1D carbon nanotubes, and 3D graphite. Various methods have been developed to produce graphene, including micromechanical exfoliation, chemical vapor deposition, and chemical synthesis. Graphene and graphene oxide (GO) have been used in biological studies due to their unique properties, such as high surface area, mechanical strength, and thermal conductivity. They have been functionalized with DNA, proteins, and other biomolecules to create biosystems for various applications, including biosensors, drug delivery, and cell imaging. Graphene-based FRET biosensors have been developed for detecting DNA, ions, small molecules, and proteins. These sensors utilize the quenching properties of graphene and the recognition capabilities of biomolecules. Graphene-based nanomaterials have shown potential in biotechnology for applications such as drug delivery, cell imaging, and biosensing. However, challenges remain in controlling the size and number of graphene layers, ensuring biocompatibility, and understanding the cellular uptake and metabolic pathways of graphene and its derivatives. Future research should focus on improving the sensitivity and specificity of graphene-based biosensors and exploring new applications in biotechnology.