This paper presents an updated inventory of Brazilian seed plants, offering insights into the country's biodiversity. The work, initiated in 2010 with the publication of the Plants and Fungi Catalogue, has been continuously updated by over 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing a 3% increase in species richness compared to 2010. The Amazon Rainforest is the richest biome for Gymnosperms, while the Atlantic Rainforest is the richest for Angiosperms. The number of species and endemism rates have increased for most biomes, except for the Amazon, which showed a 2.5% decrease in recorded endemics. Over half of Brazilian seed plant species (57.4%) are endemic to the territory. The proportion of life-forms varies among different biomes: trees are more prevalent in the Amazon and Atlantic Rainforest, herbs predominate in the Pampa, and lianas are more common in the Amazon, Atlantic Rainforest, and Pantanal. This compilation not only quantifies Brazilian biodiversity but also highlights areas where information is lacking and provides a framework for conserving the unique and diverse flora of Brazil.This paper presents an updated inventory of Brazilian seed plants, offering insights into the country's biodiversity. The work, initiated in 2010 with the publication of the Plants and Fungi Catalogue, has been continuously updated by over 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing a 3% increase in species richness compared to 2010. The Amazon Rainforest is the richest biome for Gymnosperms, while the Atlantic Rainforest is the richest for Angiosperms. The number of species and endemism rates have increased for most biomes, except for the Amazon, which showed a 2.5% decrease in recorded endemics. Over half of Brazilian seed plant species (57.4%) are endemic to the territory. The proportion of life-forms varies among different biomes: trees are more prevalent in the Amazon and Atlantic Rainforest, herbs predominate in the Pampa, and lianas are more common in the Amazon, Atlantic Rainforest, and Pantanal. This compilation not only quantifies Brazilian biodiversity but also highlights areas where information is lacking and provides a framework for conserving the unique and diverse flora of Brazil.