The article introduces GtRNAdb, a comprehensive database of transfer RNA (tRNA) genes identified in genomic sequences. The database, created by Patricia P. Chan and Todd M. Lowe, currently includes over 74,000 tRNA genes from 740 species. It provides detailed information such as tRNA gene predictions, isotype and genetic locus statistics, primary sequences, secondary structures, and multiple sequence alignments. Users can search the database by sequence similarity, tRNA characteristics, or phylogenetic group. The database also offers direct links to UCSC genome browsers for visualizing tRNA genes in the context of other genetic information. GtRNAdb is publicly available at http://gtrnadb.ucsc.edu and is a valuable resource for researchers in tRNA and genomics. The article highlights the database's features, including tRNA identification information, secondary structures, alignments, and search capabilities, as well as plans for future improvements.The article introduces GtRNAdb, a comprehensive database of transfer RNA (tRNA) genes identified in genomic sequences. The database, created by Patricia P. Chan and Todd M. Lowe, currently includes over 74,000 tRNA genes from 740 species. It provides detailed information such as tRNA gene predictions, isotype and genetic locus statistics, primary sequences, secondary structures, and multiple sequence alignments. Users can search the database by sequence similarity, tRNA characteristics, or phylogenetic group. The database also offers direct links to UCSC genome browsers for visualizing tRNA genes in the context of other genetic information. GtRNAdb is publicly available at http://gtrnadb.ucsc.edu and is a valuable resource for researchers in tRNA and genomics. The article highlights the database's features, including tRNA identification information, secondary structures, alignments, and search capabilities, as well as plans for future improvements.