The third edition of the Guide to Receptors and Channels (GRAC) aims to provide a concise and organized overview of pharmacological targets, enabling rapid identification of key properties. The guide is designed to be user-friendly, allowing newcomers to quickly grasp essential information about various receptor and channel families. It includes entries for targets with sufficient pharmacological data, focusing on human receptors where possible, and providing links to structural and pharmacological information through databases like Ensembl. When human data is unavailable, data from other species is used. The guide is divided into seven sections based on similar structure and function: 7TM receptors, transmitter-gated channels, ion channels, catalytic receptors, nuclear receptors, transporters, and enzymes. Compared to the second edition, the third edition has expanded the number of entries by nearly 20% and includes lists of 'orphan' receptors, which are targets with unknown functions. The editors compiled the entries with input from consultants and followed guidelines from the International Pharmacological Congress (NC-IUPHAR) for nomenclature. Where guidance was lacking, expert advice was sought to ensure consistency. Tabulated data allow comparison of selective agents and probes, while additional commentary highlights potential confounding factors such as species differences or ligand metabolism. Citations to the guide should follow a specific format. The guide is published with the British Journal of Pharmacology and is intended to serve as a reliable reference for pharmacological research.The third edition of the Guide to Receptors and Channels (GRAC) aims to provide a concise and organized overview of pharmacological targets, enabling rapid identification of key properties. The guide is designed to be user-friendly, allowing newcomers to quickly grasp essential information about various receptor and channel families. It includes entries for targets with sufficient pharmacological data, focusing on human receptors where possible, and providing links to structural and pharmacological information through databases like Ensembl. When human data is unavailable, data from other species is used. The guide is divided into seven sections based on similar structure and function: 7TM receptors, transmitter-gated channels, ion channels, catalytic receptors, nuclear receptors, transporters, and enzymes. Compared to the second edition, the third edition has expanded the number of entries by nearly 20% and includes lists of 'orphan' receptors, which are targets with unknown functions. The editors compiled the entries with input from consultants and followed guidelines from the International Pharmacological Congress (NC-IUPHAR) for nomenclature. Where guidance was lacking, expert advice was sought to ensure consistency. Tabulated data allow comparison of selective agents and probes, while additional commentary highlights potential confounding factors such as species differences or ligand metabolism. Citations to the guide should follow a specific format. The guide is published with the British Journal of Pharmacology and is intended to serve as a reliable reference for pharmacological research.