Serial synchrotron crystallography (SSX) is a technique that allows the determination of noncryogenic crystal structures of macromolecules while minimizing radiation damage. It is an advancement over conventional macromolecular crystallography (MX), which uses synchrotron X-rays with a single crystal. SSX offers new opportunities for structural biology research, providing a deeper understanding of macromolecule structure and function. It is particularly useful for time-resolved studies, enabling the observation of molecular reactions and dynamics. SSX uses synchrotron X-rays and has the advantage of being more accessible than X-ray free electron laser (XFEL) techniques, which are limited in number and availability. SSX can be used for both time-resolved and non-time-resolved studies, making it suitable for a wide range of applications. The technique involves the collection of diffraction data from multiple small-wedge datasets, which are then merged to determine the crystal structure. SSX also allows for the use of various sample delivery methods, including injector-based and fixed-target scanning techniques, to ensure efficient data collection. The data processing involves the identification and indexing of diffraction patterns, followed by integration and structure determination. SSX has the potential to revolutionize structural biology by providing new insights into macromolecular structures and functions.Serial synchrotron crystallography (SSX) is a technique that allows the determination of noncryogenic crystal structures of macromolecules while minimizing radiation damage. It is an advancement over conventional macromolecular crystallography (MX), which uses synchrotron X-rays with a single crystal. SSX offers new opportunities for structural biology research, providing a deeper understanding of macromolecule structure and function. It is particularly useful for time-resolved studies, enabling the observation of molecular reactions and dynamics. SSX uses synchrotron X-rays and has the advantage of being more accessible than X-ray free electron laser (XFEL) techniques, which are limited in number and availability. SSX can be used for both time-resolved and non-time-resolved studies, making it suitable for a wide range of applications. The technique involves the collection of diffraction data from multiple small-wedge datasets, which are then merged to determine the crystal structure. SSX also allows for the use of various sample delivery methods, including injector-based and fixed-target scanning techniques, to ensure efficient data collection. The data processing involves the identification and indexing of diffraction patterns, followed by integration and structure determination. SSX has the potential to revolutionize structural biology by providing new insights into macromolecular structures and functions.