The article describes the development and application of a "mitosis instead of meiosis" (MiMe) system to generate clonal gametes in hybrid tomato genotypes, enabling the creation of polyploid plants with the complete genetic repertoire of their four inbred grandparents. This approach, referred to as "4-haplotype" (4-Hap) plants, allows for precise control over genetic heterozygosity and exploitation of autopolyploid progressive heterosis (APH). The MiMe system was established in three hybrid tomato genotypes by mutating specific genes, resulting in the production of unreduced pollen. Through hybridization of these MiMe hybrids, 4-Hap plants were generated, which exhibited consistent phenotypes and maintained the genetic diversity of their parents. The study demonstrates that clonal gamete production in hybrid crops can facilitate the engineering of polyploid genomes, potentially leading to new breeding schemes and enhanced crop performance.The article describes the development and application of a "mitosis instead of meiosis" (MiMe) system to generate clonal gametes in hybrid tomato genotypes, enabling the creation of polyploid plants with the complete genetic repertoire of their four inbred grandparents. This approach, referred to as "4-haplotype" (4-Hap) plants, allows for precise control over genetic heterozygosity and exploitation of autopolyploid progressive heterosis (APH). The MiMe system was established in three hybrid tomato genotypes by mutating specific genes, resulting in the production of unreduced pollen. Through hybridization of these MiMe hybrids, 4-Hap plants were generated, which exhibited consistent phenotypes and maintained the genetic diversity of their parents. The study demonstrates that clonal gamete production in hybrid crops can facilitate the engineering of polyploid genomes, potentially leading to new breeding schemes and enhanced crop performance.