This thesis, supervised by Prof. Dr. Ana Paula Junqueira Kipnis, evaluates the effects of a homeopathic vaccine composed of *Mycobacterium massiliense* and an allopathic vaccine formulated with *Mycobacterium smegmatis* recombinant in different animal models. The study aims to assess the prophylactic effect of the homeopathic vaccine and determine its potency as a vaccine. Female BALB/c mice were divided into 15 groups, each with three animals, and were vaccinated with either the 11cH or 19cH vaccine. The 19cH vaccine induced the production of IgG2a class antibodies specific to *M. massiliense*, similar to the response observed in animals infected with the bacteria alone. For the allopathic vaccine, *M. smegmatis* mc²155 with PLA71/Fusion was used in cattle. After vaccination, blood samples were collected, and serum was analyzed using ELISA. Animals vaccinated with the recombinant live vaccine expressing the fusion protein showed higher levels of specific antibodies (p<0.01). The study concludes that both the homeopathic and allopathic vaccines, using different types of mycobacteria, can induce a humoral immune response in animal models.This thesis, supervised by Prof. Dr. Ana Paula Junqueira Kipnis, evaluates the effects of a homeopathic vaccine composed of *Mycobacterium massiliense* and an allopathic vaccine formulated with *Mycobacterium smegmatis* recombinant in different animal models. The study aims to assess the prophylactic effect of the homeopathic vaccine and determine its potency as a vaccine. Female BALB/c mice were divided into 15 groups, each with three animals, and were vaccinated with either the 11cH or 19cH vaccine. The 19cH vaccine induced the production of IgG2a class antibodies specific to *M. massiliense*, similar to the response observed in animals infected with the bacteria alone. For the allopathic vaccine, *M. smegmatis* mc²155 with PLA71/Fusion was used in cattle. After vaccination, blood samples were collected, and serum was analyzed using ELISA. Animals vaccinated with the recombinant live vaccine expressing the fusion protein showed higher levels of specific antibodies (p<0.01). The study concludes that both the homeopathic and allopathic vaccines, using different types of mycobacteria, can induce a humoral immune response in animal models.