This thesis evaluates the effects of homeopathic and allopathic vaccines against Mycobacterium spp in different animal models. The study aimed to assess the prophylactic effect of homeopathic vaccines and determine the optimal potency for use as a vaccine. It also evaluated the protective effect of an allopathic recombinant vaccine against Mycobacterium spp in cattle. The research involved experiments with mice and cattle, using Mycobacterium massiliense and Mycobacterium smegmatis as models. The homeopathic vaccine was prepared from Mycobacterium massiliense and tested in mice, showing induced production of IgG2a antibodies specific to M. massiliense. The allopathic vaccine, composed of Mycobacterium smegmatis expressing the CMX protein, demonstrated higher levels of specific antibodies in vaccinated cattle. The study concluded that both homeopathic and allopathic vaccines, using different types of mycobacteria, can induce humoral immune responses in animal models. Keywords: allopathy, homeopathy, immunity vaccine, recombinant vaccine.This thesis evaluates the effects of homeopathic and allopathic vaccines against Mycobacterium spp in different animal models. The study aimed to assess the prophylactic effect of homeopathic vaccines and determine the optimal potency for use as a vaccine. It also evaluated the protective effect of an allopathic recombinant vaccine against Mycobacterium spp in cattle. The research involved experiments with mice and cattle, using Mycobacterium massiliense and Mycobacterium smegmatis as models. The homeopathic vaccine was prepared from Mycobacterium massiliense and tested in mice, showing induced production of IgG2a antibodies specific to M. massiliense. The allopathic vaccine, composed of Mycobacterium smegmatis expressing the CMX protein, demonstrated higher levels of specific antibodies in vaccinated cattle. The study concluded that both homeopathic and allopathic vaccines, using different types of mycobacteria, can induce humoral immune responses in animal models. Keywords: allopathy, homeopathy, immunity vaccine, recombinant vaccine.