The study isolated and cultured hepatic lipocytes from normal rat liver, demonstrating their ability to produce and secrete collagen types I, III, and IV. Lipocytes were identified by their prominent rough endoplasmic reticulum and cytoplasmic droplets containing vitamin A. In primary culture, lipocytes secreted collagen types I (72.1–84.7%), type III (2.6–7.2%), and type IV (11.2–29.7%), with a total collagen content of 5% of the total protein secreted. This collagen synthesis was significantly higher than that of hepatocytes and sinusoidal endothelial cells. The findings suggest that hepatic lipocytes are the primary collagen-producing cells in normal rat liver, primarily producing type I collagen. The phenotypic resemblance of lipocytes to fibroblasts supports the hypothesis that they may be precursors of fibroblast-like cells observed in liver injury and fibrosis.The study isolated and cultured hepatic lipocytes from normal rat liver, demonstrating their ability to produce and secrete collagen types I, III, and IV. Lipocytes were identified by their prominent rough endoplasmic reticulum and cytoplasmic droplets containing vitamin A. In primary culture, lipocytes secreted collagen types I (72.1–84.7%), type III (2.6–7.2%), and type IV (11.2–29.7%), with a total collagen content of 5% of the total protein secreted. This collagen synthesis was significantly higher than that of hepatocytes and sinusoidal endothelial cells. The findings suggest that hepatic lipocytes are the primary collagen-producing cells in normal rat liver, primarily producing type I collagen. The phenotypic resemblance of lipocytes to fibroblasts supports the hypothesis that they may be precursors of fibroblast-like cells observed in liver injury and fibrosis.