Elsevier established a free COVID-19 resource center in January 2020, offering English and Mandarin information on the virus. The center allows unrestricted reuse of research in PubMed Central and other repositories. The article describes the molecular cloning and sequencing of the full-length genome of the Hepatitis E Virus (HEV) strain from Burma. The virus has a single-stranded, positive-sense RNA genome of approximately 7.5 kb with a polyadenylated 3' end. The genome contains three open reading frames (ORFs): ORF1, ORF2, and ORF3. ORF1 encodes the RNA-dependent RNA polymerase and nucleoside triphosphate binding motifs, while ORF2 contains a signal peptide and a capsid-like region. ORF3 is partially overlapping with ORF1 and ORF2. The virus is nonenveloped, with a diameter of 27–34 nm. The genetic organization and expression strategy of HEV suggest it may represent a new class of RNA virus or a separate genus within the Caliciviridae family. The study provides insights into the structure and function of HEV, including its potential role in human disease. The findings highlight the importance of HEV in the context of non-A, non-B hepatitis and its transmission through fecal-oral routes. The research also discusses the implications of HEV's genetic organization for understanding viral replication and pathogenesis. The study contributes to the broader understanding of RNA viruses and their classification.Elsevier established a free COVID-19 resource center in January 2020, offering English and Mandarin information on the virus. The center allows unrestricted reuse of research in PubMed Central and other repositories. The article describes the molecular cloning and sequencing of the full-length genome of the Hepatitis E Virus (HEV) strain from Burma. The virus has a single-stranded, positive-sense RNA genome of approximately 7.5 kb with a polyadenylated 3' end. The genome contains three open reading frames (ORFs): ORF1, ORF2, and ORF3. ORF1 encodes the RNA-dependent RNA polymerase and nucleoside triphosphate binding motifs, while ORF2 contains a signal peptide and a capsid-like region. ORF3 is partially overlapping with ORF1 and ORF2. The virus is nonenveloped, with a diameter of 27–34 nm. The genetic organization and expression strategy of HEV suggest it may represent a new class of RNA virus or a separate genus within the Caliciviridae family. The study provides insights into the structure and function of HEV, including its potential role in human disease. The findings highlight the importance of HEV in the context of non-A, non-B hepatitis and its transmission through fecal-oral routes. The research also discusses the implications of HEV's genetic organization for understanding viral replication and pathogenesis. The study contributes to the broader understanding of RNA viruses and their classification.