This pilot study investigates the impact of high-intensity interval training (HIIT) on the cardiorespiratory fitness, musculoskeletal strength, and agility of wheelchair tennis players with spinal cord injuries. Thirty participants, aged 19 to 28, were divided into an experimental group (EG) and a control group (CG). The EG underwent a 16-week HIIT program, while the CG followed a standard training regimen. Anthropometric assessments, the wheelchair yo-yo test, and hand grip evaluation were conducted before and after the intervention. The results showed a significant Time x Group interaction for the yo-yo test and hand grip evaluation, indicating marked improvements in the intervention group (p < 0.001). No significant changes were observed in the control group. The study concludes that incorporating HIIT into the daily training regime significantly enhances cardiorespiratory fitness, musculoskeletal strength, and agility in wheelchair tennis players.This pilot study investigates the impact of high-intensity interval training (HIIT) on the cardiorespiratory fitness, musculoskeletal strength, and agility of wheelchair tennis players with spinal cord injuries. Thirty participants, aged 19 to 28, were divided into an experimental group (EG) and a control group (CG). The EG underwent a 16-week HIIT program, while the CG followed a standard training regimen. Anthropometric assessments, the wheelchair yo-yo test, and hand grip evaluation were conducted before and after the intervention. The results showed a significant Time x Group interaction for the yo-yo test and hand grip evaluation, indicating marked improvements in the intervention group (p < 0.001). No significant changes were observed in the control group. The study concludes that incorporating HIIT into the daily training regime significantly enhances cardiorespiratory fitness, musculoskeletal strength, and agility in wheelchair tennis players.