High Performance Liquid Chromatography: Fallstricke und Fehlerquellen der HPLC in Bildern

High Performance Liquid Chromatography: Fallstricke und Fehlerquellen der HPLC in Bildern

March 1997 | Dr. V. R. Meyer
High Performance Liquid Chromatography (HPLC) is a widely used analytical technique in modern laboratories due to its versatility and sensitivity. However, it is also prone to various errors that can affect the reliability and accuracy of results. Dr. Veronika R. Meyer's book, "Fallstricke und Fehlerquellen der HPLC in Bildern," provides a practical guide to identifying and eliminating common errors in HPLC. The book is not a traditional textbook or troubleshooting manual but rather a collection of the most important error sources, their detection, and resolution. It includes an introductory chapter on fundamental concepts such as chromatographic and statistical principles, Van Deemter curves, and instrument construction. Each double page of the book features a diagram (chromatogram or graph) illustrating a typical error and the exact experimental conditions under which it occurs. The book also includes interesting comparisons between materials from different manufacturers and detailed studies on the influence of peak tailing and resolution on quantification errors, especially in overlapping peaks. While the book is useful as a reference for solving problems in the laboratory, the limitation of each example to a single double page sometimes fails to provide a smooth flow of the text. The book concludes with a chapter on helpful strategies for error detection and quality assurance, including topics such as equipment testing, good laboratory practice, validation, and accreditation. The book is a practical resource for anyone using HPLC in a laboratory setting.High Performance Liquid Chromatography (HPLC) is a widely used analytical technique in modern laboratories due to its versatility and sensitivity. However, it is also prone to various errors that can affect the reliability and accuracy of results. Dr. Veronika R. Meyer's book, "Fallstricke und Fehlerquellen der HPLC in Bildern," provides a practical guide to identifying and eliminating common errors in HPLC. The book is not a traditional textbook or troubleshooting manual but rather a collection of the most important error sources, their detection, and resolution. It includes an introductory chapter on fundamental concepts such as chromatographic and statistical principles, Van Deemter curves, and instrument construction. Each double page of the book features a diagram (chromatogram or graph) illustrating a typical error and the exact experimental conditions under which it occurs. The book also includes interesting comparisons between materials from different manufacturers and detailed studies on the influence of peak tailing and resolution on quantification errors, especially in overlapping peaks. While the book is useful as a reference for solving problems in the laboratory, the limitation of each example to a single double page sometimes fails to provide a smooth flow of the text. The book concludes with a chapter on helpful strategies for error detection and quality assurance, including topics such as equipment testing, good laboratory practice, validation, and accreditation. The book is a practical resource for anyone using HPLC in a laboratory setting.
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[slides and audio] High performance liquid chromatography