This study presents the development of highly conductive, transparent, and flexible PEDOT:PSS/silver nanowire (AgNW) composites using electrospinning. The fibers were electrospun on various substrates, including poly(caprolactone) (PCL), cotton fabric, and Kapton, to investigate their electrical and optical properties. The optimal fiber composition was found to be 7% v/v AgNWs with an aspect ratio of 330, resulting in a sheet resistance of 7 Ω/sq, a conductivity of 354 S/cm, and a transmittance of 77%. The fibers exhibited excellent stability under bending, with only a slight increase in sheet resistance after 1000 bending cycles. As a proof of concept, the fibers were used as electrodes in a triboelectric nanogenerator (TENG), showing superior performance compared to metal electrodes. The TENG generated a voltage of 78.83 V and a current of 7 μA, demonstrating the potential of these fibers for flexible and transparent electronics. The study highlights the potential of electrospun PEDOT:PSS/AgNW composites as a viable alternative to expensive and unsustainable materials like indium tin oxide (ITO).This study presents the development of highly conductive, transparent, and flexible PEDOT:PSS/silver nanowire (AgNW) composites using electrospinning. The fibers were electrospun on various substrates, including poly(caprolactone) (PCL), cotton fabric, and Kapton, to investigate their electrical and optical properties. The optimal fiber composition was found to be 7% v/v AgNWs with an aspect ratio of 330, resulting in a sheet resistance of 7 Ω/sq, a conductivity of 354 S/cm, and a transmittance of 77%. The fibers exhibited excellent stability under bending, with only a slight increase in sheet resistance after 1000 bending cycles. As a proof of concept, the fibers were used as electrodes in a triboelectric nanogenerator (TENG), showing superior performance compared to metal electrodes. The TENG generated a voltage of 78.83 V and a current of 7 μA, demonstrating the potential of these fibers for flexible and transparent electronics. The study highlights the potential of electrospun PEDOT:PSS/AgNW composites as a viable alternative to expensive and unsustainable materials like indium tin oxide (ITO).