The Hippo pathway is a conserved signaling pathway essential for regulating organ growth in *Drosophila* and vertebrates. While the core kinases Hippo/Mst and Warts/Lats have been well-studied, less is known about upstream inputs and downstream outputs. This review discusses recent findings that reveal dynamic regulation of Hippo signaling during development and its diverse functions in growth control and regeneration. Key upstream regulators and their roles, as well as the pathway's interaction with other global signals, are explored. The review also highlights the complex regulatory network of the Hippo pathway, including its involvement in adult homeostasis and abnormal growth. Additionally, it examines the role of specific components such as Fat, Ex, Mer, Kibra, Crumbs, Lethal giant larvae, and Ajuba in modulating Hippo signaling. The tissue-specific differences in upstream regulation and the spatial regulation of mammalian Hippo signaling are also discussed.The Hippo pathway is a conserved signaling pathway essential for regulating organ growth in *Drosophila* and vertebrates. While the core kinases Hippo/Mst and Warts/Lats have been well-studied, less is known about upstream inputs and downstream outputs. This review discusses recent findings that reveal dynamic regulation of Hippo signaling during development and its diverse functions in growth control and regeneration. Key upstream regulators and their roles, as well as the pathway's interaction with other global signals, are explored. The review also highlights the complex regulatory network of the Hippo pathway, including its involvement in adult homeostasis and abnormal growth. Additionally, it examines the role of specific components such as Fat, Ex, Mer, Kibra, Crumbs, Lethal giant larvae, and Ajuba in modulating Hippo signaling. The tissue-specific differences in upstream regulation and the spatial regulation of mammalian Hippo signaling are also discussed.