This study summarizes the research trends and hotspots of microglia in Alzheimer's disease (AD) from 2000 to 2022 using bibliometric analysis. A total of 7449 publications were analyzed, showing a steady increase in research output over the years, with the United States publishing the most articles. The Journal of Alzheimer's Disease and Journal of Neuroscience were the most cited journals. Key authors and institutions, such as Mt Heneka and Harvard Medical School, played significant roles in the field. The most common keywords included neuroinflammation, amyloid-beta, inflammation, and neurodegeneration, while emerging hotspots included gut microbiota, extracellular vesicles, and meta-analysis. Research hotspots included NLRP3 inflammasome, TREM2, gut microbiota, mitochondrial dysfunction, and exosomes. The study highlights the role of microglia-mediated neuroinflammation in AD pathogenesis and the importance of understanding the relationship between microglia and AD. The research also emphasizes the potential of targeting these hotspots for future therapeutic strategies. The study concludes that microglia research in AD is a rapidly growing field with significant implications for understanding and treating the disease.This study summarizes the research trends and hotspots of microglia in Alzheimer's disease (AD) from 2000 to 2022 using bibliometric analysis. A total of 7449 publications were analyzed, showing a steady increase in research output over the years, with the United States publishing the most articles. The Journal of Alzheimer's Disease and Journal of Neuroscience were the most cited journals. Key authors and institutions, such as Mt Heneka and Harvard Medical School, played significant roles in the field. The most common keywords included neuroinflammation, amyloid-beta, inflammation, and neurodegeneration, while emerging hotspots included gut microbiota, extracellular vesicles, and meta-analysis. Research hotspots included NLRP3 inflammasome, TREM2, gut microbiota, mitochondrial dysfunction, and exosomes. The study highlights the role of microglia-mediated neuroinflammation in AD pathogenesis and the importance of understanding the relationship between microglia and AD. The research also emphasizes the potential of targeting these hotspots for future therapeutic strategies. The study concludes that microglia research in AD is a rapidly growing field with significant implications for understanding and treating the disease.