The article introduces Human Splicing Finder (HSF), an online bioinformatics tool designed to predict the effects of mutations on splicing signals and identify splicing motifs in human sequences. HSF includes all available matrices for auxiliary sequence prediction and new matrices for binding sites of specific proteins involved in splicing. The tool evaluates the strength of 5' and 3' splice sites and branch points using Position Weight Matrices. The efficiency of HSF was evaluated using a set of 83 intronic and 35 exonic mutations known to cause splicing defects, showing that it correctly predicted the mutation effects in almost all cases. HSF is valuable for research, diagnostics, and therapeutic purposes, particularly in studies related to alternative splicing and genetic diseases.The article introduces Human Splicing Finder (HSF), an online bioinformatics tool designed to predict the effects of mutations on splicing signals and identify splicing motifs in human sequences. HSF includes all available matrices for auxiliary sequence prediction and new matrices for binding sites of specific proteins involved in splicing. The tool evaluates the strength of 5' and 3' splice sites and branch points using Position Weight Matrices. The efficiency of HSF was evaluated using a set of 83 intronic and 35 exonic mutations known to cause splicing defects, showing that it correctly predicted the mutation effects in almost all cases. HSF is valuable for research, diagnostics, and therapeutic purposes, particularly in studies related to alternative splicing and genetic diseases.