The Human Protein Reference Database (HPRD) has been updated to include over 20,000 protein entries, making it the largest database for literature-derived protein-protein interactions and post-translational modifications (PTMs) for human proteins. New features include protein isoforms, enhanced search options, pathway annotations, and integration of the GenProt Viewer, a novel browser that integrates genomic, transcriptomic, and proteomic information. HPRD currently contains 33,710 unique protein-protein interactions and 8,409 recorded PTMs. The database also provides detailed annotations on subcellular localization and tissue expression. Future plans include developing a Protein Distributed Annotation System and integrating transcriptomic data. HPRD aims to serve as a comprehensive resource for biomedical research, facilitating the integration of genomic, transcriptomic, and proteomic data to advance biomedical discoveries.The Human Protein Reference Database (HPRD) has been updated to include over 20,000 protein entries, making it the largest database for literature-derived protein-protein interactions and post-translational modifications (PTMs) for human proteins. New features include protein isoforms, enhanced search options, pathway annotations, and integration of the GenProt Viewer, a novel browser that integrates genomic, transcriptomic, and proteomic information. HPRD currently contains 33,710 unique protein-protein interactions and 8,409 recorded PTMs. The database also provides detailed annotations on subcellular localization and tissue expression. Future plans include developing a Protein Distributed Annotation System and integrating transcriptomic data. HPRD aims to serve as a comprehensive resource for biomedical research, facilitating the integration of genomic, transcriptomic, and proteomic data to advance biomedical discoveries.