Hyaluronic acid (HA) is a biocompatible polysaccharide with wide applications in biomedical fields. HA can be chemically modified to form various forms, including hydrogels, which are used in cell therapy, regenerative medicine, and tissue engineering. These hydrogels are crosslinked using various methods, such as addition/condensation chemistry, radical polymerization, and photochemical reactions. The crosslinking chemistry must be compatible with cell encapsulation and tissue injection, and must meet regulatory and financial requirements for clinical use. HA-derived hydrogels have shown promise in delivering cells and therapeutic agents for tissue repair and regeneration. Recent advances in HA hydrogel development include the use of thiol-modified HA, haloacetate-modified HA, dihydrazide-modified HA, aldehyde-modified HA, tyramine-modified HA, and Huisgen cycloaddition (click chemistry). These hydrogels have been applied in cell delivery, molecule delivery, cell expansion and recovery, drug evaluation, and tissue engineering. HA hydrogels have also been used in cartilage tissue engineering, cardiac repair, valvular engineering, and stem cell behavior control. The mechanical properties and degradation rates of HA hydrogels can be tailored by adjusting the crosslinking density, molecular weight, and other parameters. HA hydrogels have shown potential for clinical applications in regenerative medicine, including tissue repair, drug delivery, and stem cell therapy. The development of HA hydrogels continues to advance, with new methods and applications being explored for biomedical use.Hyaluronic acid (HA) is a biocompatible polysaccharide with wide applications in biomedical fields. HA can be chemically modified to form various forms, including hydrogels, which are used in cell therapy, regenerative medicine, and tissue engineering. These hydrogels are crosslinked using various methods, such as addition/condensation chemistry, radical polymerization, and photochemical reactions. The crosslinking chemistry must be compatible with cell encapsulation and tissue injection, and must meet regulatory and financial requirements for clinical use. HA-derived hydrogels have shown promise in delivering cells and therapeutic agents for tissue repair and regeneration. Recent advances in HA hydrogel development include the use of thiol-modified HA, haloacetate-modified HA, dihydrazide-modified HA, aldehyde-modified HA, tyramine-modified HA, and Huisgen cycloaddition (click chemistry). These hydrogels have been applied in cell delivery, molecule delivery, cell expansion and recovery, drug evaluation, and tissue engineering. HA hydrogels have also been used in cartilage tissue engineering, cardiac repair, valvular engineering, and stem cell behavior control. The mechanical properties and degradation rates of HA hydrogels can be tailored by adjusting the crosslinking density, molecular weight, and other parameters. HA hydrogels have shown potential for clinical applications in regenerative medicine, including tissue repair, drug delivery, and stem cell therapy. The development of HA hydrogels continues to advance, with new methods and applications being explored for biomedical use.