This review article discusses the development and biomedical applications of hydrogels based on polysaccharides modified with carbonyl groups. The introduction of carbonyl groups into the polysaccharide backbone enhances the stability and biocompatibility of the hydrogels, making them suitable for various biomedical applications such as tissue engineering, drug delivery, and wound healing. The article highlights the mechanisms by which oxidized polysaccharides introduce carbonyl groups, leading to cross-linking with proteins. These hydrogels exhibit tunable mechanical properties and improved biocompatibility, making them promising biomaterials. The review covers the dynamic properties of these hydrogels, including their ability to form microparticles, nanoparticles, and films, and their applications in different biomedical contexts. Key topics include the chemical modifications of polysaccharides, the functional and structural characteristics of carbonyl-functionalized polysaccharides, and the controlled gelation and swelling behavior of these materials. The article also provides examples of specific polysaccharides, such as alginate, chitosan, pullulan, carboxymethylcellulose, and pectin, and their oxidation methods and properties.This review article discusses the development and biomedical applications of hydrogels based on polysaccharides modified with carbonyl groups. The introduction of carbonyl groups into the polysaccharide backbone enhances the stability and biocompatibility of the hydrogels, making them suitable for various biomedical applications such as tissue engineering, drug delivery, and wound healing. The article highlights the mechanisms by which oxidized polysaccharides introduce carbonyl groups, leading to cross-linking with proteins. These hydrogels exhibit tunable mechanical properties and improved biocompatibility, making them promising biomaterials. The review covers the dynamic properties of these hydrogels, including their ability to form microparticles, nanoparticles, and films, and their applications in different biomedical contexts. Key topics include the chemical modifications of polysaccharides, the functional and structural characteristics of carbonyl-functionalized polysaccharides, and the controlled gelation and swelling behavior of these materials. The article also provides examples of specific polysaccharides, such as alginate, chitosan, pullulan, carboxymethylcellulose, and pectin, and their oxidation methods and properties.