The article presents the INFOGEST static in vitro simulation of gastrointestinal food digestion, a standardized protocol developed by the COST INFOGEST network. This method aims to simulate the digestion of food in the upper gastrointestinal tract using constant ratios of meal to digestive fluids and a constant pH for each step. The protocol is designed to be simple and accessible, making it suitable for a wide range of researchers. It involves sequential oral, gastric, and intestinal digestion, with parameters based on physiological data. The method is useful for assessing the release of nutrients and micronutrients from food and has been widely used for various purposes, including the evaluation of protein, lipid, and starch digestion. The INFOGEST method has been compared to in vivo digestion studies, showing good correlation in protein degradation and peptide patterns. However, it has limitations in simulating dynamic processes such as pH gradients and gastric emptying. The article also discusses alternative methods and provides detailed instructions for preparing reagents, performing the digestion procedure, and analyzing samples.The article presents the INFOGEST static in vitro simulation of gastrointestinal food digestion, a standardized protocol developed by the COST INFOGEST network. This method aims to simulate the digestion of food in the upper gastrointestinal tract using constant ratios of meal to digestive fluids and a constant pH for each step. The protocol is designed to be simple and accessible, making it suitable for a wide range of researchers. It involves sequential oral, gastric, and intestinal digestion, with parameters based on physiological data. The method is useful for assessing the release of nutrients and micronutrients from food and has been widely used for various purposes, including the evaluation of protein, lipid, and starch digestion. The INFOGEST method has been compared to in vivo digestion studies, showing good correlation in protein degradation and peptide patterns. However, it has limitations in simulating dynamic processes such as pH gradients and gastric emptying. The article also discusses alternative methods and provides detailed instructions for preparing reagents, performing the digestion procedure, and analyzing samples.