The passage describes the detection and characterization of SHC fusion proteins and the identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma (KS). The methods used include PCR, cloning, and immunoprecipitation to purify and analyze the proteins and DNA sequences. The specific activity of the GST-SHC fusion proteins was measured, and their presence was confirmed through SDS-PAGE and autoradiography. The 330-bp and 631-bp sequences (KS330Bam and KS631Bam) were identified and found to have homology to herpesviral polypeptides. Southern blot hybridization and PCR amplification were used to detect these sequences in KS tissues, with a significant association observed in AIDS-KS tissues compared to non-KS tissues. The sequences were not found in control tissues, suggesting their specific association with KS. The study also tested multiple unaffected tissue samples from AIDS-KS patients, confirming that the sequences were not heritable polymorphic markers. The results indicate that these sequences may represent a new human herpesvirus associated with KS, but a causal link cannot be established.The passage describes the detection and characterization of SHC fusion proteins and the identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma (KS). The methods used include PCR, cloning, and immunoprecipitation to purify and analyze the proteins and DNA sequences. The specific activity of the GST-SHC fusion proteins was measured, and their presence was confirmed through SDS-PAGE and autoradiography. The 330-bp and 631-bp sequences (KS330Bam and KS631Bam) were identified and found to have homology to herpesviral polypeptides. Southern blot hybridization and PCR amplification were used to detect these sequences in KS tissues, with a significant association observed in AIDS-KS tissues compared to non-KS tissues. The sequences were not found in control tissues, suggesting their specific association with KS. The study also tested multiple unaffected tissue samples from AIDS-KS patients, confirming that the sequences were not heritable polymorphic markers. The results indicate that these sequences may represent a new human herpesvirus associated with KS, but a causal link cannot be established.