The article describes a platform for targeted glycosylation, called SUGAR-TARGET, which enables controlled and tailored N-linked glycosylation in vitro using immobilized enzymes. The platform addresses the limitations of existing glycoengineering techniques by mimicking a natural glycosylation pathway with promiscuous enzymes, allowing for the production of near-homogeneous glycoforms. The authors developed a one-step immobilization/purification method for glycosyltransferases, enabling sequential reactions in an in vitro environment. They demonstrated the versatility of SUGAR-TARGET by successfully applying it to free glycans, recombinant glycoproteins from glycoengineered hosts, and full-length IgGs. The platform showed high conversion rates and homogeneity in glycoform production, with significant improvements in galactosylation profiles of IgGs. Additionally, the immobilized GalT demonstrated reusability over multiple cycles, highlighting its potential for large-scale industrial applications. The study highlights the potential of SUGAR-TARGET for generating tailored glycoforms and advancing the field of glycoengineering.The article describes a platform for targeted glycosylation, called SUGAR-TARGET, which enables controlled and tailored N-linked glycosylation in vitro using immobilized enzymes. The platform addresses the limitations of existing glycoengineering techniques by mimicking a natural glycosylation pathway with promiscuous enzymes, allowing for the production of near-homogeneous glycoforms. The authors developed a one-step immobilization/purification method for glycosyltransferases, enabling sequential reactions in an in vitro environment. They demonstrated the versatility of SUGAR-TARGET by successfully applying it to free glycans, recombinant glycoproteins from glycoengineered hosts, and full-length IgGs. The platform showed high conversion rates and homogeneity in glycoform production, with significant improvements in galactosylation profiles of IgGs. Additionally, the immobilized GalT demonstrated reusability over multiple cycles, highlighting its potential for large-scale industrial applications. The study highlights the potential of SUGAR-TARGET for generating tailored glycoforms and advancing the field of glycoengineering.