This study presents an improved amplification method for genital human papillomaviruses (HPVs) using the PGMY09/11 primer system, which enhances sensitivity and reproducibility compared to the standard MY09/11 system. The MY09/11 primers, which amplify a broad range of HPV genotypes, had limitations in sensitivity due to mismatches between primer sequences and target HPV types. The PGMY09/11 system was developed by designing multiple primer sequences that avoid degenerate bases, allowing for more consistent and sensitive amplification across a wide range of HPV types. This new system was tested on 262 cervicovaginal lavage specimens and showed a 91.5% overall agreement with the MY09/11 system, with a higher prevalence of HPV infections (62.8% vs. 55.1%). The PGMY09/11 system detected additional HPV-positive specimens and increased the proportion of multiple infections detected. It also showed improved sensitivity for several HPV types, including cancer-associated types such as 26, 35, 45, 52, 55, 59, 68, 73, and MM7. The PGMY09/11 system is more sensitive and reproducible, making it a valuable tool for HPV typing and studying the natural history of HPV infections. The study highlights the importance of improving HPV detection methods to better understand the role of HPV in cervical cancer and other genital diseases.This study presents an improved amplification method for genital human papillomaviruses (HPVs) using the PGMY09/11 primer system, which enhances sensitivity and reproducibility compared to the standard MY09/11 system. The MY09/11 primers, which amplify a broad range of HPV genotypes, had limitations in sensitivity due to mismatches between primer sequences and target HPV types. The PGMY09/11 system was developed by designing multiple primer sequences that avoid degenerate bases, allowing for more consistent and sensitive amplification across a wide range of HPV types. This new system was tested on 262 cervicovaginal lavage specimens and showed a 91.5% overall agreement with the MY09/11 system, with a higher prevalence of HPV infections (62.8% vs. 55.1%). The PGMY09/11 system detected additional HPV-positive specimens and increased the proportion of multiple infections detected. It also showed improved sensitivity for several HPV types, including cancer-associated types such as 26, 35, 45, 52, 55, 59, 68, 73, and MM7. The PGMY09/11 system is more sensitive and reproducible, making it a valuable tool for HPV typing and studying the natural history of HPV infections. The study highlights the importance of improving HPV detection methods to better understand the role of HPV in cervical cancer and other genital diseases.