A re-evaluation of the Longin collagen-extraction method shows that lower reflux temperatures reduce protein degradation, allowing better purification through ultrafiltration to isolate the >30kDalton fraction. This modification improves the yield of larger peptides compared to the original method. The study used three bones of different ages and tested the effects of reflux temperature and acid concentration on product yield and molecular weight distribution. Results showed that refluxing at 58°C maximizes solubilization while minimizing degradation. The >30kDalton fraction, obtained through ultrafiltration, is more suitable for radiocarbon dating as it contains fewer contaminants. The modified method involves demineralizing bone, refluxing in weak acid at 58°C, and ultrafiltrating to isolate the >30kDalton fraction. This method produces non-hygroscopic products that are easier to handle and provide more reliable radiocarbon dates. The study concludes that the modified Longin method improves collagen extraction and yields more accurate radiocarbon dates. The results suggest that the >30kDalton fraction is more reliable for dating than the traditional Longin product. The study was supported by funding from NSERC and universities. References to previous studies are included.A re-evaluation of the Longin collagen-extraction method shows that lower reflux temperatures reduce protein degradation, allowing better purification through ultrafiltration to isolate the >30kDalton fraction. This modification improves the yield of larger peptides compared to the original method. The study used three bones of different ages and tested the effects of reflux temperature and acid concentration on product yield and molecular weight distribution. Results showed that refluxing at 58°C maximizes solubilization while minimizing degradation. The >30kDalton fraction, obtained through ultrafiltration, is more suitable for radiocarbon dating as it contains fewer contaminants. The modified method involves demineralizing bone, refluxing in weak acid at 58°C, and ultrafiltrating to isolate the >30kDalton fraction. This method produces non-hygroscopic products that are easier to handle and provide more reliable radiocarbon dates. The study concludes that the modified Longin method improves collagen extraction and yields more accurate radiocarbon dates. The results suggest that the >30kDalton fraction is more reliable for dating than the traditional Longin product. The study was supported by funding from NSERC and universities. References to previous studies are included.