The Pathosystems Resource Integration Center (PATRIC) is an all-bacterial Bioinformatics Resource Center that has undergone significant improvements, including a redesigned web interface and new services. The new interface enhances user access to tools and data, with a focus on user-created genome groups and detailed summaries. Key enhancements include: 1. **Enhanced Private Data Analysis**: Researchers can now assemble and annotate raw sequence reads using RASTtk, and perform RNA-Seq, variation, model reconstruction, and differential expression analysis within a private workspace. Private data can be compared to public data through 'virtual integration.' 2. **Expanded Genome Collection**: PATRIC now includes over 80,000 genomes, with a special emphasis on genomes with antimicrobial resistance (AMR) data. This data is used to improve subsystem annotation and k-mer classification, and to predict antibiotic susceptibility or resistance. 3. **New Services**: PATRIC offers a range of services such as genome assembly, annotation, RNA-Seq analysis, expression import, proteome comparison, metabolic model reconstruction, and variation analysis. These services are designed to streamline common bioinformatic workflows and are accessible through a private workspace. 4. **Data Enhancements**: PATRIC imports genomes from GenBank monthly and has assembled approximately 8,500 genomes with AMR metadata from SRA and ENA. The platform also maintains up-to-date protein families and provides downloadable files for all genomes, annotations, and AMR metadata. 5. **Future Directions**: PATRIC plans to enhance clinical research support, increase focus on modeling, and improve therapeutics support by leveraging advanced computational approaches and multi-omics data integration. These improvements aim to provide a more comprehensive and user-friendly platform for bacterial genomics and bioinformatics research.The Pathosystems Resource Integration Center (PATRIC) is an all-bacterial Bioinformatics Resource Center that has undergone significant improvements, including a redesigned web interface and new services. The new interface enhances user access to tools and data, with a focus on user-created genome groups and detailed summaries. Key enhancements include: 1. **Enhanced Private Data Analysis**: Researchers can now assemble and annotate raw sequence reads using RASTtk, and perform RNA-Seq, variation, model reconstruction, and differential expression analysis within a private workspace. Private data can be compared to public data through 'virtual integration.' 2. **Expanded Genome Collection**: PATRIC now includes over 80,000 genomes, with a special emphasis on genomes with antimicrobial resistance (AMR) data. This data is used to improve subsystem annotation and k-mer classification, and to predict antibiotic susceptibility or resistance. 3. **New Services**: PATRIC offers a range of services such as genome assembly, annotation, RNA-Seq analysis, expression import, proteome comparison, metabolic model reconstruction, and variation analysis. These services are designed to streamline common bioinformatic workflows and are accessible through a private workspace. 4. **Data Enhancements**: PATRIC imports genomes from GenBank monthly and has assembled approximately 8,500 genomes with AMR metadata from SRA and ENA. The platform also maintains up-to-date protein families and provides downloadable files for all genomes, annotations, and AMR metadata. 5. **Future Directions**: PATRIC plans to enhance clinical research support, increase focus on modeling, and improve therapeutics support by leveraging advanced computational approaches and multi-omics data integration. These improvements aim to provide a more comprehensive and user-friendly platform for bacterial genomics and bioinformatics research.