This study evaluates the in vitro safety and functional probiotic properties of the novel *Bacillus coagulans* strain CGI314. The strain was assessed for its resistance to heat, gastric acid, and bile salts, adhesion to intestinal cells, auto-aggregation and co-aggregation properties, antibacterial activity, metabolite production, antioxidant capacity, cholesterol assimilation, and anti-inflammatory activity. The results show that *B. coagulans* CGI314 spores are highly resistant to high temperatures and gastric and intestinal conditions, demonstrating their ability to survive under gastrointestinal conditions. The strain also exhibited moderate auto-aggregation and co-aggregation with potential pathogens. Metabolomic profiling revealed the synthesis of several amino acids, vitamins, and short-chain fatty acids. Additionally, *B. coagulans* CGI314 demonstrated strong antioxidant capacity and cytoprotective effects against oxidative stress in HT-29 and HepG2 cell lines. The strain also showed cholesterol-reducing ability and antagonistic activity against various pathogens. Overall, these findings suggest that *B. coagulans* CGI314 has promising probiotic potential and could be a valuable candidate for functional foods.This study evaluates the in vitro safety and functional probiotic properties of the novel *Bacillus coagulans* strain CGI314. The strain was assessed for its resistance to heat, gastric acid, and bile salts, adhesion to intestinal cells, auto-aggregation and co-aggregation properties, antibacterial activity, metabolite production, antioxidant capacity, cholesterol assimilation, and anti-inflammatory activity. The results show that *B. coagulans* CGI314 spores are highly resistant to high temperatures and gastric and intestinal conditions, demonstrating their ability to survive under gastrointestinal conditions. The strain also exhibited moderate auto-aggregation and co-aggregation with potential pathogens. Metabolomic profiling revealed the synthesis of several amino acids, vitamins, and short-chain fatty acids. Additionally, *B. coagulans* CGI314 demonstrated strong antioxidant capacity and cytoprotective effects against oxidative stress in HT-29 and HepG2 cell lines. The strain also showed cholesterol-reducing ability and antagonistic activity against various pathogens. Overall, these findings suggest that *B. coagulans* CGI314 has promising probiotic potential and could be a valuable candidate for functional foods.