This study investigates the inducible isoforms of cyclooxygenase (COX) and nitric oxide synthase (NOS) in a murine air pouch model of granulomatous inflammation. COX and NOS activities were measured in skin samples during the acute phase (up to 24 hours) and in granulomatous tissue at various stages of chronic and resolving inflammation (3, 5, 7, 14, and 21 days). COX-1 and COX-2 proteins were assessed by Western blot. The results show that COX activity in the skin increased over the first 24 hours and continued to rise up to day 14, with COX-2 protein levels peaking at day 14. COX-1 protein levels remained unchanged. iNOS activity increased in the skin over the first 24 hours and then showed a significant increase in granulomatous tissue between days 3 and 7, followed by a decrease at day 14 and another increase at day 21. The findings suggest that COX and NOS activities in the skin during the acute phase reinforce their proinflammatory roles, but in the chronic and resolving stages, there is a dissociation between COX and NOS activities, possibly due to the changing pattern of cytokines during the inflammatory response.This study investigates the inducible isoforms of cyclooxygenase (COX) and nitric oxide synthase (NOS) in a murine air pouch model of granulomatous inflammation. COX and NOS activities were measured in skin samples during the acute phase (up to 24 hours) and in granulomatous tissue at various stages of chronic and resolving inflammation (3, 5, 7, 14, and 21 days). COX-1 and COX-2 proteins were assessed by Western blot. The results show that COX activity in the skin increased over the first 24 hours and continued to rise up to day 14, with COX-2 protein levels peaking at day 14. COX-1 protein levels remained unchanged. iNOS activity increased in the skin over the first 24 hours and then showed a significant increase in granulomatous tissue between days 3 and 7, followed by a decrease at day 14 and another increase at day 21. The findings suggest that COX and NOS activities in the skin during the acute phase reinforce their proinflammatory roles, but in the chronic and resolving stages, there is a dissociation between COX and NOS activities, possibly due to the changing pattern of cytokines during the inflammatory response.