The pH of the wound environment plays a crucial role in wound healing, a complex process involving the degradation and reassembly of connective tissue and epidermal layers. While it has long been assumed that a low pH, like that of normal skin, is beneficial for healing, recent studies show that some healing processes, such as skin graft take-rate, require an alkaline environment. This review discusses the impact of wound pH on healing and highlights the need for further research to understand pH requirements under different conditions. The pH of normal skin ranges from 4 to 6 and is important for skin barrier function and resistance to external chemicals. In wounds, the pH becomes more alkaline due to exposure of underlying tissue with a pH of 7.4. This change can affect microbial growth, as many pathogens thrive in alkaline conditions. However, the presence of bacteria in wounds may delay healing, particularly for Staphylococcus aureus. The body's natural defenses, such as neutrophils, can lower wound pH to combat infection. The pH also influences the effectiveness of antibiotics, with some becoming more active in alkaline environments. In chronic wounds, the pH can affect proteolytic activity, which is crucial for tissue repair. The review emphasizes the need for more systematic data to develop pH-targeted therapeutic strategies for wound healing. Understanding the role of pH in wound healing could lead to more effective treatments for chronic wounds and improve patient outcomes.The pH of the wound environment plays a crucial role in wound healing, a complex process involving the degradation and reassembly of connective tissue and epidermal layers. While it has long been assumed that a low pH, like that of normal skin, is beneficial for healing, recent studies show that some healing processes, such as skin graft take-rate, require an alkaline environment. This review discusses the impact of wound pH on healing and highlights the need for further research to understand pH requirements under different conditions. The pH of normal skin ranges from 4 to 6 and is important for skin barrier function and resistance to external chemicals. In wounds, the pH becomes more alkaline due to exposure of underlying tissue with a pH of 7.4. This change can affect microbial growth, as many pathogens thrive in alkaline conditions. However, the presence of bacteria in wounds may delay healing, particularly for Staphylococcus aureus. The body's natural defenses, such as neutrophils, can lower wound pH to combat infection. The pH also influences the effectiveness of antibiotics, with some becoming more active in alkaline environments. In chronic wounds, the pH can affect proteolytic activity, which is crucial for tissue repair. The review emphasizes the need for more systematic data to develop pH-targeted therapeutic strategies for wound healing. Understanding the role of pH in wound healing could lead to more effective treatments for chronic wounds and improve patient outcomes.