Dengue, a severe zoonotic viral infection transmitted by the *Aedes* mosquito, is a significant global health concern, causing approximately 25,000 deaths annually. The resurgence of dengue is attributed to climate change, demographic transitions, and evolving social dynamics. Developing an effective vaccine against dengue has been challenging due to the presence of four distinct viral serotypes with different antigenic profiles. This review emphasizes the urgent need to address the dengue threat through the application of biotechnological and omics sciences, in conjunction with the "One Health" approach, which promotes collaborative surveillance and research across various disciplines. The review highlights the impact of climate change on dengue transmission, the expansion of *Aedes* mosquito vectors, and the challenges posed by pesticide resistance. It also discusses the current state of dengue vaccines, vector control strategies, and diagnostic methods. The integration of omics sciences, such as genomics, proteomics, and metabolomics, is crucial for developing new diagnostic techniques and prophylactic interventions. The review concludes by emphasizing the importance of a multidisciplinary approach to manage the threat of dengue effectively.Dengue, a severe zoonotic viral infection transmitted by the *Aedes* mosquito, is a significant global health concern, causing approximately 25,000 deaths annually. The resurgence of dengue is attributed to climate change, demographic transitions, and evolving social dynamics. Developing an effective vaccine against dengue has been challenging due to the presence of four distinct viral serotypes with different antigenic profiles. This review emphasizes the urgent need to address the dengue threat through the application of biotechnological and omics sciences, in conjunction with the "One Health" approach, which promotes collaborative surveillance and research across various disciplines. The review highlights the impact of climate change on dengue transmission, the expansion of *Aedes* mosquito vectors, and the challenges posed by pesticide resistance. It also discusses the current state of dengue vaccines, vector control strategies, and diagnostic methods. The integration of omics sciences, such as genomics, proteomics, and metabolomics, is crucial for developing new diagnostic techniques and prophylactic interventions. The review concludes by emphasizing the importance of a multidisciplinary approach to manage the threat of dengue effectively.