The article discusses the integration of One Health strategies in managing Dengue, a severe zoonotic disease transmitted by Aedes mosquitoes. Dengue is a major global health concern, with increasing incidence due to climate change, urbanization, and globalization. The One Health approach emphasizes collaboration among human, animal, and environmental health sectors to address the disease effectively. The review highlights the role of biotechnology and -OMICS sciences in developing new strategies for Dengue control. Climate change influences mosquito distribution and viral transmission, increasing the risk of Dengue. The article also discusses the challenges of managing Dengue, including pesticide resistance and the need for effective vector control. Current vaccines, such as Dengvaxia and Qdenga, are tetravalent and target all four Dengue serotypes. The review emphasizes the importance of integrating -OMICS sciences with One Health strategies to develop new diagnostic tools, vaccines, and control measures. The article concludes that a multidisciplinary approach is essential for addressing the growing threat of Dengue.The article discusses the integration of One Health strategies in managing Dengue, a severe zoonotic disease transmitted by Aedes mosquitoes. Dengue is a major global health concern, with increasing incidence due to climate change, urbanization, and globalization. The One Health approach emphasizes collaboration among human, animal, and environmental health sectors to address the disease effectively. The review highlights the role of biotechnology and -OMICS sciences in developing new strategies for Dengue control. Climate change influences mosquito distribution and viral transmission, increasing the risk of Dengue. The article also discusses the challenges of managing Dengue, including pesticide resistance and the need for effective vector control. Current vaccines, such as Dengvaxia and Qdenga, are tetravalent and target all four Dengue serotypes. The review emphasizes the importance of integrating -OMICS sciences with One Health strategies to develop new diagnostic tools, vaccines, and control measures. The article concludes that a multidisciplinary approach is essential for addressing the growing threat of Dengue.