This study investigates the intracellular dynamics of hippocampal place cells during virtual navigation in head-restrained mice. Using a virtual reality system, mice ran on a spherical treadmill while interacting with a computer-generated environment. Whole-cell recordings combined with virtual navigation allowed researchers to examine the subthreshold membrane potential dynamics of place cells, revealing three key features: (1) an asymmetric ramp-like depolarization of the baseline membrane potential, (2) increased amplitude of intracellular theta oscillations, and (3) phase precession of intracellular theta relative to extracellular theta. These dynamics underlie the primary features of place cell rate and temporal coding. The virtual reality system enables new experimental approaches to study the neural circuits underlying navigation. The study also demonstrates that head-restrained mice can perform visually-guided spatial behaviors in a virtual environment, with place cells showing firing rate and phase precession characteristics similar to those observed in freely-moving mice. Intracellular recordings revealed that place cells exhibit ramp-like depolarizations of the baseline membrane potential and modulations of theta oscillations, which are consistent with the soma-dendritic interference model of hippocampal coding. The virtual reality system allows for precise control of the environment and enables the study of intracellular dynamics in head-restrained mice, providing insights into the mechanisms of spatial navigation. The findings suggest that the virtual reality system can facilitate new experiments to explore spatial information encoding and the cellular and synaptic mechanisms underlying hippocampal coding.This study investigates the intracellular dynamics of hippocampal place cells during virtual navigation in head-restrained mice. Using a virtual reality system, mice ran on a spherical treadmill while interacting with a computer-generated environment. Whole-cell recordings combined with virtual navigation allowed researchers to examine the subthreshold membrane potential dynamics of place cells, revealing three key features: (1) an asymmetric ramp-like depolarization of the baseline membrane potential, (2) increased amplitude of intracellular theta oscillations, and (3) phase precession of intracellular theta relative to extracellular theta. These dynamics underlie the primary features of place cell rate and temporal coding. The virtual reality system enables new experimental approaches to study the neural circuits underlying navigation. The study also demonstrates that head-restrained mice can perform visually-guided spatial behaviors in a virtual environment, with place cells showing firing rate and phase precession characteristics similar to those observed in freely-moving mice. Intracellular recordings revealed that place cells exhibit ramp-like depolarizations of the baseline membrane potential and modulations of theta oscillations, which are consistent with the soma-dendritic interference model of hippocampal coding. The virtual reality system allows for precise control of the environment and enables the study of intracellular dynamics in head-restrained mice, providing insights into the mechanisms of spatial navigation. The findings suggest that the virtual reality system can facilitate new experiments to explore spatial information encoding and the cellular and synaptic mechanisms underlying hippocampal coding.