The article introduces EzBioCloud, an integrated database that combines taxonomic information with 16S rRNA gene sequences and whole-genome assemblies of *Bacteria* and *Archaea*. The database aims to enhance the classification and identification of prokaryotic species by providing a comprehensive taxonomic hierarchy. It includes 61,700 species/phylogroups and 62,362 whole-genome assemblies, identified at the genus, species, and subspecies levels. The database uses a composite bioinformatics pipeline that combines gene-based searches and average nucleotide identity (ANI) calculations to ensure accurate taxonomic assignments. Additionally, it provides genomic properties such as genome size, DNA G+C content, and occurrence in human microbiome data. The database and its search tools are accessible at www.ezbiocloud.net/ and are expected to accelerate genome-based classification and identification in microbiology.The article introduces EzBioCloud, an integrated database that combines taxonomic information with 16S rRNA gene sequences and whole-genome assemblies of *Bacteria* and *Archaea*. The database aims to enhance the classification and identification of prokaryotic species by providing a comprehensive taxonomic hierarchy. It includes 61,700 species/phylogroups and 62,362 whole-genome assemblies, identified at the genus, species, and subspecies levels. The database uses a composite bioinformatics pipeline that combines gene-based searches and average nucleotide identity (ANI) calculations to ensure accurate taxonomic assignments. Additionally, it provides genomic properties such as genome size, DNA G+C content, and occurrence in human microbiome data. The database and its search tools are accessible at www.ezbiocloud.net/ and are expected to accelerate genome-based classification and identification in microbiology.