The article discusses the transition from 2D to 3D cell culture in biomedical research. 2D cell culture has been the standard for decades but has limitations in accurately representing the complex in vivo environment. 3D cell culture techniques offer more accurate models of cell behavior, including cell-to-cell interactions, tumor characteristics, and drug responses. Various 3D culture methods, such as scaffold-based techniques (hydrogels, polymeric scaffolds, hydrophilic glass fibers, organoids) and scaffold-free techniques (hanging drop microplates, magnetic levitation, spheroid microplates), are discussed. 3D cell culture has potential applications in drug discovery, cancer research, stem cell studies, and disease modeling. 3D models can provide more accurate data than 2D models, especially in drug screening and metabolic profiling. 3D cell culture also allows for the study of organ behavior through organoids and may eventually replace animal models. The article highlights the advantages of 3D cell culture over 2D, including better mimicry of in vivo environments, improved drug response predictions, and enhanced metabolic profiling. 3D cell culture is also beneficial for stem cell research, as it allows for the study of cell differentiation and tissue regeneration. The article concludes that 3D cell culture is a promising alternative to 2D cell culture and has the potential to revolutionize biomedical research.The article discusses the transition from 2D to 3D cell culture in biomedical research. 2D cell culture has been the standard for decades but has limitations in accurately representing the complex in vivo environment. 3D cell culture techniques offer more accurate models of cell behavior, including cell-to-cell interactions, tumor characteristics, and drug responses. Various 3D culture methods, such as scaffold-based techniques (hydrogels, polymeric scaffolds, hydrophilic glass fibers, organoids) and scaffold-free techniques (hanging drop microplates, magnetic levitation, spheroid microplates), are discussed. 3D cell culture has potential applications in drug discovery, cancer research, stem cell studies, and disease modeling. 3D models can provide more accurate data than 2D models, especially in drug screening and metabolic profiling. 3D cell culture also allows for the study of organ behavior through organoids and may eventually replace animal models. The article highlights the advantages of 3D cell culture over 2D, including better mimicry of in vivo environments, improved drug response predictions, and enhanced metabolic profiling. 3D cell culture is also beneficial for stem cell research, as it allows for the study of cell differentiation and tissue regeneration. The article concludes that 3D cell culture is a promising alternative to 2D cell culture and has the potential to revolutionize biomedical research.