This study investigates the transport of RNA in neurons, focusing on the role of kinesin and the formation of RNA-transporting granules. The authors isolated a large RNase-sensitive granule (1000S−) that binds to conventional kinesin (KIF5) and identified 42 proteins associated with this granule, including mRNAs for CaMKIIα and Arc. Seventeen of these proteins were further characterized, revealing their classification, binding combinations, and their role in RNA transport. The granules were found to colocalize with KIF5 and RNA in dendrites, and their movement was bidirectional, with distally directed movement enhanced by KIF5 overexpression and reduced by its functional blockage. This suggests that kinesin transports RNA via these granules in a coordinated manner with opposite motors like dynein. The study also highlights the importance of specific proteins in RNA transport and provides insights into the mechanisms of RNA transport in neurons.This study investigates the transport of RNA in neurons, focusing on the role of kinesin and the formation of RNA-transporting granules. The authors isolated a large RNase-sensitive granule (1000S−) that binds to conventional kinesin (KIF5) and identified 42 proteins associated with this granule, including mRNAs for CaMKIIα and Arc. Seventeen of these proteins were further characterized, revealing their classification, binding combinations, and their role in RNA transport. The granules were found to colocalize with KIF5 and RNA in dendrites, and their movement was bidirectional, with distally directed movement enhanced by KIF5 overexpression and reduced by its functional blockage. This suggests that kinesin transports RNA via these granules in a coordinated manner with opposite motors like dynein. The study also highlights the importance of specific proteins in RNA transport and provides insights into the mechanisms of RNA transport in neurons.