This study investigates the kinetics of periodate-mediated oxidation of cellulose to dialdehyde cellulose (DAC). The reaction was modeled using a pseudo-first-order approach to determine rate-limiting steps and overall reaction kinetics. Experimental data were used to calculate rate constants for DAC formation (k₁), cellulose degradation (k₂), and DAC degradation (k₃). The results showed that temperature had a more significant effect on k₁ than periodate concentration, while k₂ and k₃ were less affected by periodate concentration but increased with temperature. The oxidation reaction followed pseudo-first-order kinetics, with higher temperatures leading to faster DAC formation and degradation. The Arrhenius equation was used to determine temperature-dependent intrinsic kinetic constants, revealing activation energies of 30.4 kJ/mol, 72.27 kJ/mol, and 23.9 kJ/mol for k₁₀, k₂₀, and k₃₀, respectively. These findings provide valuable insights for optimizing the oxidation process to achieve higher DAC yields with reduced reaction times and periodate concentrations. The study highlights the importance of balancing temperature and periodate concentration to maximize DAC production while minimizing degradation. Kinetic modeling is essential for understanding the reaction mechanism and improving the efficiency of cellulose oxidation processes. The results have implications for the development of sustainable cellulose-based materials and their applications in various fields, including biomedical and environmental technologies.This study investigates the kinetics of periodate-mediated oxidation of cellulose to dialdehyde cellulose (DAC). The reaction was modeled using a pseudo-first-order approach to determine rate-limiting steps and overall reaction kinetics. Experimental data were used to calculate rate constants for DAC formation (k₁), cellulose degradation (k₂), and DAC degradation (k₃). The results showed that temperature had a more significant effect on k₁ than periodate concentration, while k₂ and k₃ were less affected by periodate concentration but increased with temperature. The oxidation reaction followed pseudo-first-order kinetics, with higher temperatures leading to faster DAC formation and degradation. The Arrhenius equation was used to determine temperature-dependent intrinsic kinetic constants, revealing activation energies of 30.4 kJ/mol, 72.27 kJ/mol, and 23.9 kJ/mol for k₁₀, k₂₀, and k₃₀, respectively. These findings provide valuable insights for optimizing the oxidation process to achieve higher DAC yields with reduced reaction times and periodate concentrations. The study highlights the importance of balancing temperature and periodate concentration to maximize DAC production while minimizing degradation. Kinetic modeling is essential for understanding the reaction mechanism and improving the efficiency of cellulose oxidation processes. The results have implications for the development of sustainable cellulose-based materials and their applications in various fields, including biomedical and environmental technologies.