Lambda ZAP is a bacteriophage lambda expression vector with in vivo excision properties. This vector allows for the excision of a phagemid containing DNA inserts from the lambda vector, enabling the rapid transfer of DNA from lambda to plasmid cloning vectors. The excision process is facilitated by the f1 phage origin of replication, which contains both initiation and termination signals for DNA synthesis. The vector incorporates these signals, allowing for the excision of the phagemid pBluescript SK(-) from the lambda vector, which can then be used for further analysis. The vector was tested by constructing a chicken liver cDNA library and isolating actin clones using DNA or antibody probes. The ability of Lambda ZAP to serve as a vector for the construction of cDNA expression libraries was determined by detecting fusion proteins from clones containing glucocerbrosidase cDNA using rabbit IgG anti-glucocerbrosidase antibodies. The vector was also used to construct a cDNA clone expressing a human glucocerebrosidase gene, which was verified by antibody screening. The vector offers several advantages, including the ability to rapidly excise DNA inserts and the presence of multiple restriction sites for cloning. The vector is suitable for the construction of genomic libraries and can be used as a cDNA expression vector. The in vivo excision process allows for the rapid analysis of cloned genes and the construction of high-efficiency lambda libraries instead of standard plasmid libraries. The vector is also useful for the construction of jumping or junction libraries and for cloning strategies that avoid DNA methylation. The vector has a large insert capacity and is suitable for the construction of genomic libraries. The vector was constructed by inserting plasmid sequences into a nonessential region of a lambda phage, flanked by the initiator and terminator domains. The vector was tested for its excision efficiency and was found to be effective in excising DNA inserts. The vector was also used to construct a cDNA library and to isolate actin and glucocerebrosidase clones. The vector was found to be useful for rapid sequence analysis and for the construction of genomic libraries. The vector is suitable for the construction of cDNA expression libraries and for the analysis of cloned genes. The vector was also used to construct a cDNA clone expressing a human glucocerebrosidase gene, which was verified by antibody screening. The vector offers several advantages, including the ability to rapidly excise DNA inserts and the presence of multiple restriction sites for cloning. The vector is suitable for the construction of genomic libraries and can be used as a cDNA expression vector. The in vivo excision process allows for the rapid analysis of cloned genes and the construction of high-efficiency lambda libraries instead of standard plasmid libraries. The vector is also useful for the construction of jumping or junction libraries and for cloning strategies that avoid DNA methylation. The vector has a large insert capacity and is suitable for theLambda ZAP is a bacteriophage lambda expression vector with in vivo excision properties. This vector allows for the excision of a phagemid containing DNA inserts from the lambda vector, enabling the rapid transfer of DNA from lambda to plasmid cloning vectors. The excision process is facilitated by the f1 phage origin of replication, which contains both initiation and termination signals for DNA synthesis. The vector incorporates these signals, allowing for the excision of the phagemid pBluescript SK(-) from the lambda vector, which can then be used for further analysis. The vector was tested by constructing a chicken liver cDNA library and isolating actin clones using DNA or antibody probes. The ability of Lambda ZAP to serve as a vector for the construction of cDNA expression libraries was determined by detecting fusion proteins from clones containing glucocerbrosidase cDNA using rabbit IgG anti-glucocerbrosidase antibodies. The vector was also used to construct a cDNA clone expressing a human glucocerebrosidase gene, which was verified by antibody screening. The vector offers several advantages, including the ability to rapidly excise DNA inserts and the presence of multiple restriction sites for cloning. The vector is suitable for the construction of genomic libraries and can be used as a cDNA expression vector. The in vivo excision process allows for the rapid analysis of cloned genes and the construction of high-efficiency lambda libraries instead of standard plasmid libraries. The vector is also useful for the construction of jumping or junction libraries and for cloning strategies that avoid DNA methylation. The vector has a large insert capacity and is suitable for the construction of genomic libraries. The vector was constructed by inserting plasmid sequences into a nonessential region of a lambda phage, flanked by the initiator and terminator domains. The vector was tested for its excision efficiency and was found to be effective in excising DNA inserts. The vector was also used to construct a cDNA library and to isolate actin and glucocerebrosidase clones. The vector was found to be useful for rapid sequence analysis and for the construction of genomic libraries. The vector is suitable for the construction of cDNA expression libraries and for the analysis of cloned genes. The vector was also used to construct a cDNA clone expressing a human glucocerebrosidase gene, which was verified by antibody screening. The vector offers several advantages, including the ability to rapidly excise DNA inserts and the presence of multiple restriction sites for cloning. The vector is suitable for the construction of genomic libraries and can be used as a cDNA expression vector. The in vivo excision process allows for the rapid analysis of cloned genes and the construction of high-efficiency lambda libraries instead of standard plasmid libraries. The vector is also useful for the construction of jumping or junction libraries and for cloning strategies that avoid DNA methylation. The vector has a large insert capacity and is suitable for the