A light-controlled soft bio-microrobot (Ebot) based on Euglena gracilis is developed for biomedical applications. The Ebot can perform multiple tasks in narrow microenvironments, including targeted drug delivery, selective removal of diseased cells in intestinal mucosa, and photodynamic therapy (PDT). The motion of the Ebot is controlled by light-controlled polygonal flagellum beating, and its deformability is regulated by different light illumination durations, allowing it to navigate through narrow and curved microchannels. The Ebot's ability to deform and adapt to different environments enables it to execute various biomedical tasks. The Ebot's motion can be precisely controlled by varying light intensity, and its deformation can be controlled by varying light irradiation duration. The Ebot can pass through different narrow and complicated microfluidic channels, demonstrating its adaptability. The Ebot can also be used for targeted drug delivery and selective removal of diseased cells in cell clusters. The Ebot's photosynthetic capability allows it to be used for PDT, where it can generate reactive oxygen species (ROS) under red light irradiation, leading to the death of cancer cells. The Ebot is biocompatible and biodegradable, making it suitable for biomedical applications. The Ebot's motion and deformation are controlled by light, and it can be used for in vivo applications with the help of biomedical optical fibers for light delivery. The Ebot can be coated with mammalian neutrophil membrane to reduce immune rejection or encapsulated in degradable oral capsules for in vivo applications. The Ebot has the potential for various biomedical tasks in narrow and complicated environments where conventional tools are difficult to access.A light-controlled soft bio-microrobot (Ebot) based on Euglena gracilis is developed for biomedical applications. The Ebot can perform multiple tasks in narrow microenvironments, including targeted drug delivery, selective removal of diseased cells in intestinal mucosa, and photodynamic therapy (PDT). The motion of the Ebot is controlled by light-controlled polygonal flagellum beating, and its deformability is regulated by different light illumination durations, allowing it to navigate through narrow and curved microchannels. The Ebot's ability to deform and adapt to different environments enables it to execute various biomedical tasks. The Ebot's motion can be precisely controlled by varying light intensity, and its deformation can be controlled by varying light irradiation duration. The Ebot can pass through different narrow and complicated microfluidic channels, demonstrating its adaptability. The Ebot can also be used for targeted drug delivery and selective removal of diseased cells in cell clusters. The Ebot's photosynthetic capability allows it to be used for PDT, where it can generate reactive oxygen species (ROS) under red light irradiation, leading to the death of cancer cells. The Ebot is biocompatible and biodegradable, making it suitable for biomedical applications. The Ebot's motion and deformation are controlled by light, and it can be used for in vivo applications with the help of biomedical optical fibers for light delivery. The Ebot can be coated with mammalian neutrophil membrane to reduce immune rejection or encapsulated in degradable oral capsules for in vivo applications. The Ebot has the potential for various biomedical tasks in narrow and complicated environments where conventional tools are difficult to access.