The article describes a novel method for rapid DNA sequence analysis using light-generated oligonucleotide arrays. The authors present a technique that combines photolithographic techniques with combinatorial chemistry to create miniaturized arrays of densely packed oligonucleotide probes, known as DNA chips. These arrays can be used for parallel DNA hybridization analysis, directly providing sequence information. In a preliminary experiment, a 1.28 × 1.28 cm array of 256 different octanucleotides was synthesized in 16 chemical reaction cycles, taking 4 hours to complete. The hybridization pattern of fluorescently labeled oligonucleotide targets was detected by epifluorescence microscopy, demonstrating high specificity and sensitivity. The method is expected to be a powerful tool for rapid investigations in human genetics, diagnostics, pathogen detection, and DNA molecular recognition. The authors also discuss the potential applications of these arrays in genome sequencing projects, genetic diagnostic testing, and the study of RNA- or protein-DNA interactions.The article describes a novel method for rapid DNA sequence analysis using light-generated oligonucleotide arrays. The authors present a technique that combines photolithographic techniques with combinatorial chemistry to create miniaturized arrays of densely packed oligonucleotide probes, known as DNA chips. These arrays can be used for parallel DNA hybridization analysis, directly providing sequence information. In a preliminary experiment, a 1.28 × 1.28 cm array of 256 different octanucleotides was synthesized in 16 chemical reaction cycles, taking 4 hours to complete. The hybridization pattern of fluorescently labeled oligonucleotide targets was detected by epifluorescence microscopy, demonstrating high specificity and sensitivity. The method is expected to be a powerful tool for rapid investigations in human genetics, diagnostics, pathogen detection, and DNA molecular recognition. The authors also discuss the potential applications of these arrays in genome sequencing projects, genetic diagnostic testing, and the study of RNA- or protein-DNA interactions.