This review discusses the role of long non-coding RNAs (lncRNAs) in regulating cell signaling pathways, particularly in cancer. LncRNAs have been identified as key regulators of gene expression through various mechanisms, including transcriptional and posttranscriptional regulation. They can interact with DNA, RNA, and proteins to modulate chromatin structure, mRNA stability, splicing, and translation. The review highlights the involvement of lncRNAs in important cell signaling pathways such as p53, NF-κB, PI3K/AKT, and Notch. For example, lncRNAs like DINO and Linc-RoR play crucial roles in p53 regulation, while lncRNAs such as NKILA and PACER are involved in NF-κB signaling. In the PI3K/AKT pathway, lncRNAs like AK023948 and LINK-A regulate AKT activity. Additionally, lncRNAs associated with the Notch signaling pathway, such as LUNAR1 and TUG1, are essential for stem cell maintenance and cancer progression. The review also discusses the impact of lncRNAs on Wnt/β-catenin and HIF1α signaling pathways. Overall, lncRNAs contribute to various aspects of tumorigenesis by regulating these signaling pathways, and their dysregulation may lead to cancer development and progression.This review discusses the role of long non-coding RNAs (lncRNAs) in regulating cell signaling pathways, particularly in cancer. LncRNAs have been identified as key regulators of gene expression through various mechanisms, including transcriptional and posttranscriptional regulation. They can interact with DNA, RNA, and proteins to modulate chromatin structure, mRNA stability, splicing, and translation. The review highlights the involvement of lncRNAs in important cell signaling pathways such as p53, NF-κB, PI3K/AKT, and Notch. For example, lncRNAs like DINO and Linc-RoR play crucial roles in p53 regulation, while lncRNAs such as NKILA and PACER are involved in NF-κB signaling. In the PI3K/AKT pathway, lncRNAs like AK023948 and LINK-A regulate AKT activity. Additionally, lncRNAs associated with the Notch signaling pathway, such as LUNAR1 and TUG1, are essential for stem cell maintenance and cancer progression. The review also discusses the impact of lncRNAs on Wnt/β-catenin and HIF1α signaling pathways. Overall, lncRNAs contribute to various aspects of tumorigenesis by regulating these signaling pathways, and their dysregulation may lead to cancer development and progression.