A low-friction soft robot (LFSR) was developed for targeted bacterial infection treatment in the gastrointestinal (GI) tract. The robot is composed of densely arranged cone structures and hydrophobic monolayers, enabling it to move with reduced drag and improved velocity in non-Newtonian liquids. The robot's stiffness can be reversibly controlled using a magnetic field, allowing for on-demand actuation and removal of bacterial films. Additionally, the magnetocaloric effect can be used to eradicate bacteria through high-frequency alternating magnetic fields (AMF). Clinical imaging-guided actuation platforms were developed to enable vision-based control and delivery of the robots. The LFSR's low surface friction and adaptability allow it to navigate the GI tract's complex terrain, including plicae gastricae obstacles. The robot can be used for mechanical scratching of biofilms and magnetocaloric heating to kill bacteria. The LFSR's motion performance was tested in various environments, including mucus, and it showed excellent adaptability and maneuverability. The robot was also tested in a stomach phantom with randomly distributed plicae gastricae, demonstrating its ability to navigate through obstacles. The LFSR was shown to effectively remove bacterial films and debris from the GI tract. The robot's motion was tracked using US and x-ray imaging, demonstrating its potential for targeted bacterial infection therapy. The LFSR's use of magnetocaloric effect for bacterial eradication was also demonstrated, showing its effectiveness in killing bacteria. The LFSR's ability to navigate the GI tract and perform targeted bacterial infection therapy makes it a promising solution for treating bacterial infections in the body.A low-friction soft robot (LFSR) was developed for targeted bacterial infection treatment in the gastrointestinal (GI) tract. The robot is composed of densely arranged cone structures and hydrophobic monolayers, enabling it to move with reduced drag and improved velocity in non-Newtonian liquids. The robot's stiffness can be reversibly controlled using a magnetic field, allowing for on-demand actuation and removal of bacterial films. Additionally, the magnetocaloric effect can be used to eradicate bacteria through high-frequency alternating magnetic fields (AMF). Clinical imaging-guided actuation platforms were developed to enable vision-based control and delivery of the robots. The LFSR's low surface friction and adaptability allow it to navigate the GI tract's complex terrain, including plicae gastricae obstacles. The robot can be used for mechanical scratching of biofilms and magnetocaloric heating to kill bacteria. The LFSR's motion performance was tested in various environments, including mucus, and it showed excellent adaptability and maneuverability. The robot was also tested in a stomach phantom with randomly distributed plicae gastricae, demonstrating its ability to navigate through obstacles. The LFSR was shown to effectively remove bacterial films and debris from the GI tract. The robot's motion was tracked using US and x-ray imaging, demonstrating its potential for targeted bacterial infection therapy. The LFSR's use of magnetocaloric effect for bacterial eradication was also demonstrated, showing its effectiveness in killing bacteria. The LFSR's ability to navigate the GI tract and perform targeted bacterial infection therapy makes it a promising solution for treating bacterial infections in the body.