This research article presents a three-dimensional scaffold inspired by the lymph node environment to enhance the therapeutic effects of chimeric antigen receptor (CAR)-T cells in solid tumors. The scaffold, made from poly(lactic-co-glycolic acid) using microfluidic techniques, is designed to load, deliver, activate, and expand CAR-T cells. It is modified with T-cell stimulatory signals, including anti-CD3 and anti-CD28 antibodies, as well as cytokines. The scaffold demonstrates significant improvements in CAR-T cell expansion both in vitro (50-fold) and in vivo (15-fold). Notably, it maintains long-lasting expansion of CAR-T cells for up to 30 days in a cervical tumor model, significantly inhibiting tumor growth. This biomimetic delivery strategy provides a versatile platform for enhancing the effectiveness of CAR-T cell therapy in solid tumors.This research article presents a three-dimensional scaffold inspired by the lymph node environment to enhance the therapeutic effects of chimeric antigen receptor (CAR)-T cells in solid tumors. The scaffold, made from poly(lactic-co-glycolic acid) using microfluidic techniques, is designed to load, deliver, activate, and expand CAR-T cells. It is modified with T-cell stimulatory signals, including anti-CD3 and anti-CD28 antibodies, as well as cytokines. The scaffold demonstrates significant improvements in CAR-T cell expansion both in vitro (50-fold) and in vivo (15-fold). Notably, it maintains long-lasting expansion of CAR-T cells for up to 30 days in a cervical tumor model, significantly inhibiting tumor growth. This biomimetic delivery strategy provides a versatile platform for enhancing the effectiveness of CAR-T cell therapy in solid tumors.