Lymphatic vessels, part of the secondary circulatory system, play a key role in maintaining homeostasis in tissues and organs. They are responsible for lymph absorption, transport, and immune surveillance. Lymphatic vessel development involves complex signaling pathways that guide heterogeneous cell populations to form specialized networks. Lymphangiogenesis, the formation of new lymphatic vessels from existing ones, is crucial for embryonic lymphatic network development. Abnormal lymphangiogenesis is linked to diseases like lymphedema, obesity, and cancer. Mechanistic studies have identified potential targets for regulating abnormal lymphangiogenesis, which could improve disease treatment. Clinical trials show promise in using these targets for therapeutic interventions. Lymphatic vessels originate from various cell populations, including venous endothelial cells and mesenchymal cells, and their development is regulated by factors like VEGFC, VEGFR3, and PROX1. Lymphatic vessel development involves stages such as progenitor cell specification, migration, and maturation. Lymphatic vessels are essential for fluid and lipid transport, immune cell trafficking, and maintaining homeostasis. They also play a role in disease processes, such as Alzheimer's disease, by affecting the clearance of pathological proteins. Lymphatic vessels in different organs, including the brain, eyes, heart, and intestines, have distinct functions and developmental pathways. Lymphatic vessels are involved in immune responses by transporting antigens and immune cells to lymph nodes, where they are processed and presented. Lymphatic vessels also contribute to the clearance of cerebrospinal fluid and aqueous humor, and their dysfunction can lead to neurological disorders. Overall, understanding the development, function, and regulation of lymphatic vessels is crucial for developing therapeutic strategies for diseases involving abnormal lymphangiogenesis.Lymphatic vessels, part of the secondary circulatory system, play a key role in maintaining homeostasis in tissues and organs. They are responsible for lymph absorption, transport, and immune surveillance. Lymphatic vessel development involves complex signaling pathways that guide heterogeneous cell populations to form specialized networks. Lymphangiogenesis, the formation of new lymphatic vessels from existing ones, is crucial for embryonic lymphatic network development. Abnormal lymphangiogenesis is linked to diseases like lymphedema, obesity, and cancer. Mechanistic studies have identified potential targets for regulating abnormal lymphangiogenesis, which could improve disease treatment. Clinical trials show promise in using these targets for therapeutic interventions. Lymphatic vessels originate from various cell populations, including venous endothelial cells and mesenchymal cells, and their development is regulated by factors like VEGFC, VEGFR3, and PROX1. Lymphatic vessel development involves stages such as progenitor cell specification, migration, and maturation. Lymphatic vessels are essential for fluid and lipid transport, immune cell trafficking, and maintaining homeostasis. They also play a role in disease processes, such as Alzheimer's disease, by affecting the clearance of pathological proteins. Lymphatic vessels in different organs, including the brain, eyes, heart, and intestines, have distinct functions and developmental pathways. Lymphatic vessels are involved in immune responses by transporting antigens and immune cells to lymph nodes, where they are processed and presented. Lymphatic vessels also contribute to the clearance of cerebrospinal fluid and aqueous humor, and their dysfunction can lead to neurological disorders. Overall, understanding the development, function, and regulation of lymphatic vessels is crucial for developing therapeutic strategies for diseases involving abnormal lymphangiogenesis.