This study presents a novel non-enzymatic glucose sensor based on a Cu₂O/M/AC nanocomposite, where MXene is embedded in a porous carbon matrix. The composite was synthesized using the coprecipitation method and characterized using techniques such as FESEM, XRD, and electrochemical methods. The porous structure of activated carbon facilitated the incorporation of MXene and Cu₂O, enhancing charge transfer and providing a larger surface area for glucose sensing. The composite exhibited two linear ranges for glucose detection with a limit of detection (LOD) of 1.96 μM. The linear ranges were 0.004 to 13.3 mM and 15.3 to 28.4 mM, with sensitivities of 430.3 and 240.5 μA mM⁻¹ cm⁻², respectively. The composite showed good sensitivity, selectivity, and a low LOD, making it suitable for non-enzymatic glucose sensing. The study also demonstrated the composite's ability to detect glucose in human serum with a 99% recovery rate, indicating its potential for real-time applications. The MXene-embedded porous carbon-based Cu₂O nanocomposite offers a wide linear range, high sensitivity, and good selectivity, making it a promising candidate for non-enzymatic glucose sensors.This study presents a novel non-enzymatic glucose sensor based on a Cu₂O/M/AC nanocomposite, where MXene is embedded in a porous carbon matrix. The composite was synthesized using the coprecipitation method and characterized using techniques such as FESEM, XRD, and electrochemical methods. The porous structure of activated carbon facilitated the incorporation of MXene and Cu₂O, enhancing charge transfer and providing a larger surface area for glucose sensing. The composite exhibited two linear ranges for glucose detection with a limit of detection (LOD) of 1.96 μM. The linear ranges were 0.004 to 13.3 mM and 15.3 to 28.4 mM, with sensitivities of 430.3 and 240.5 μA mM⁻¹ cm⁻², respectively. The composite showed good sensitivity, selectivity, and a low LOD, making it suitable for non-enzymatic glucose sensing. The study also demonstrated the composite's ability to detect glucose in human serum with a 99% recovery rate, indicating its potential for real-time applications. The MXene-embedded porous carbon-based Cu₂O nanocomposite offers a wide linear range, high sensitivity, and good selectivity, making it a promising candidate for non-enzymatic glucose sensors.